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HYPOXIA EFFECTS ON TRYPANOSOMA CRUZI EPIMASTIGOTES PROLIFERATION, DIFFERENTIATION, AND ENERGY METABOLISM
Bioenergética
Espécies que reagem ao oxigênio
Trypanosoma cruzi
Metabolismo do parasita
Autor
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Resumen en ingles
Trypanosoma cruzi, the causative agent of Chagas disease, faces changes in redox status and
nutritional availability during its life cycle. However, the influence of oxygen fluctuation upon the
biology of T. cruzi is unclear. The present work investigated the response of T. cruzi epimastigotes
to hypoxia. The parasites showed an adaptation to the hypoxic condition, presenting an increase
in proliferation and a reduction in metacyclogenesis. Additionally, parasites cultured in hypoxia
produced more reactive oxygen species (ROS) compared to parasites cultured in normoxia. The
analyses of the mitochondrial physiology demonstrated that hypoxic condition induced a decrease
in both oxidative phosphorylation and mitochondrial membrane potential (DYm) in epimastigotes.
In spite of that, ATP levels of parasites cultivated in hypoxia increased. The hypoxic condition
also increased the expression of the hexokinase and NADH fumarate reductase genes and reduced
NAD(P)H, suggesting that this increase in ATP levels of hypoxia-challenged parasites was a consequence
of increased glycolysis and fermentation pathways. Taken together, our results suggest
that decreased oxygen levels trigger a shift in the bioenergetic metabolism of T. cruzi epimastigotes,
favoring ROS production and fermentation to sustain ATP production, allowing the parasite to
survive and proliferate in the insect vector.
Palabras clave en portugues
HipóxiaBioenergética
Espécies que reagem ao oxigênio
Trypanosoma cruzi
Metabolismo do parasita
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