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DEVELOPMENT OF A LABORATORIAL PLATFORM FOR DIAGNOSIS OF SCHISTOSOMIASIS MANSONI BY PCR-ELISA
Souza, Carolina Senra Alves de et al. | Fecha del documento: 2018
Autor
Souza, Carolina Senra Alves de
Gomes, Luciana Inácia
Siqueira, Liliane Maria Vidal
Coelho, Paulo Marcos Zech
Rabello, Ana Lúcia Teles
Oliveira, Edward José de
Afiliación
Clinical Research and Public Politics in Infectious and Parasitic Diseases. Institute René Rachou. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil.
Clinical Research and Public Politics in Infectious and Parasitic Diseases. Institute René Rachou. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil.
Biology of the Schistosoma mansoni and its Interaction with Host. Institute René Rachou. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil.
Biology of the Schistosoma mansoni and its Interaction with Host. Institute René Rachou. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil.
Clinical Research and Public Politics in Infectious and Parasitic Diseases. Institute René Rachou. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil.
Clinical Research and Public Politics in Infectious and Parasitic Diseases. Institute René Rachou. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil.
Resumen en ingles
Objective: We developed a laboratorial platform to release a commercial platform used in the PCR-ELISA for the molecular diagnosis of schistosomiasis mansoni. On following, PCR-ELISA platform laboratorial was evaluated in 206 feces samples collected of individual living in a Brazilian low endemicity area. Results: The PCR-ELISA laboratorial platform indicated a prevalence rate of 25.2%, which was higher than the Kato-Katz technique (18.4%) and lower than the commercial platform (30.1%). Considering Kato-Katz technique as the reference, there were 97.4% and 91.1% of relative sensitivity and specificity rates, respectively. The laboratorial platform presented good precision, performance diagnostic, and can be used in replacement to the commercial platform for diagnosis of schistosomiasis by PCR-ELISA.
Palabras clave en ingles
Diagnosis
Disease control
PCR-ELISA
Schistosoma mansoni
Schistosomiasis
 
Editor
Biomed Central
Referencia
SOUZA, Carolina Senra Alves de et al. Development of a laboratorial platform for diagnosis of schistosomiasis mansoni by PCR-ELISA. BMC Res Notes, v. 11, n. 1, 455, 2018. doi: 10.1186/s13104-018-3571-7.
DOI
10.1186/s13104-018-3571-7
ISSN
1756-0500