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TOXICITY OF SPIKE FRAGMENTS SARS-COV-2 S PROTEIN FOR ZEBRAFISH: A TOOL TO STUDY ITS HAZARDOUS FOR HUMAN HEALTH?
Danio rerio
Environmental impacts
Infection diseases
Acute respiratory syndrome
Fausto K. Ferraris. Oswaldo Cruz Foundation. Institute Oswaldo Cruz. Department of Pharmacology and Toxicology. Rio de Janeiro, RJ, Brazil.
Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
a Laboratório de Controle Genético e Sanitário, Diretoria Técnica de Apoio ao Ensino e Pesquisa, Faculdade de Medicina da Universidade de São Paulo, Brazil b Laboratório Integrado de Biociências Translacionais (LIBT), Instituto de Biodiversidade e Sustentabilidade (NUPEM), Universidade Federal do Rio de Janeiro (UFRJ), Macaé, RJ, Brazil c Department of Microbiology, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, Brazil d Department of Cell Biology, Institute of Biomedical Sciences, University of São Paulo, Brazil e Reproductive and Molecular Biology Group, Department of Morphology, Institute of Biosciences, Sao Paulo State University, Botucatu, São Paulo, Brazil f Department of Structural and Functional Biology, Institute of Biosciences of Botucatu, São Paulo State University, SP, Brazil g Department of Preventive Veterinary Medicine, São Paulo State University (UNESP), Jaboticabal, Brazil h Department of Pharmacology, Center of Research in Inflammatory Diseases, Ribeirao Preto Medical School, University of São Paulo, Brazil i Brasil University, Descalvado, São Paulo, Brazil j Universidade Estadual Paulista Júlio de Mesquita Filho, Instituto de Biociências - Departamento de Fisiologia, São Paulo, Brazil k Universidade Estadual Paulista Júlio de Mesquita Filho, São Paulo, Brazil l Postgraduate Program in Health Sciences, PROCISA, Federal University of Roraima, Brazil m Immunochemistry Laboratory, Butantan Institute, São Paulo, Brazil n Aquaculture Health Research and Extension Group, GRUPESA, Aquaculture Health Laboratory, LABSA, Department of Veterinary Medicine, Federal University of Rondônia, Rolim de Moura campus, Rondônia, Brazil o Department of Preventive Veterinary Medicine, São Paulo State University, Jaboticabal, Brazil p Department of Cell and Molecular Biology, Ribeirao Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil q Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Brazil r Pontifícia Universidade Católica de Minas Gerais, Brazil s Departamento de Ciências Biomoleculares, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil t Department of Pharmacology and Toxicology, Oswaldo Cruz Foundation, FIOCRUZ, Rio de Janeiro, Brazil u Department of Biochemistry and Immunology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Brazil v Laboratory of Physiology, INCQS/Fiocruz Zebrafish Facility, Departament of Pharmacology and Toxicology, National Institute for Quality Control in Health, Brasil w Transplantation Immunobiology Lab, Department of Immunology, Institute of Biomedical Sciences, Universidade de Sao Paulo, Brazil x Center for Translational Research in Oncology, Cancer Institute of the State of Sao Paulo, Faculty of Medicine, University of São Paulo, Sao Paulo, Brazil y Laboratory of Peptide Biochemistry, Federal University of São Paulo, Brazil z Laboratory of Human Immunology, Department Immunology, Institute Biomedical Sciences, University São Paulo, Sao Paulo, Brazil aa Department of Pharmacology, Universidade Federal de Minas Gerais, Brazil ab Graduate Program of Pharmacology, Federal University of Santa Maria, Brazil ac Laboratory of Fish Physiology, Graduate Program of Bioexperimentation and of Environmental Sciences, University of Passo Fundo, Brazil.
Author
Affilliation
Abstract
Despite the significant increase in the generation of SARS-CoV-2 contaminated domestic and hospital wastewater, little is known about the ecotoxicological effects of the virus or its structural components in freshwater vertebrates. Inthis context, this study evaluated the deleterious effects caused by SARS-CoV-2 Spike protein on the health of Daniorerio, zebrafish. We demonstrated, for the first time, that zebrafish injected with fragment 16 to 165 (rSpike), whichcorresponds to the N-terminal portion of the protein, presented mortalities and adverse effects on liver, kidney,ovary and brain tissues. The conserved genetic homology between zebrafish and humans might be one of the reasonsfor the intense toxic effects followed inflammatory reaction from the immune system of zebrafish to rSpike which provoked damage to organs in a similar pattern as happen in severe cases of COVID-19 in humans, and, resulted in 78,6%of survival rate in female adults during the first seven days. The application of spike protein in zebrafish was highlytoxic that is suitable for future studies to gather valuable information about ecotoxicological impacts, as well as vaccineresponses and therapeutic approaches in human medicine. Therefore, besides representing an important tool to assessthe harmful effects of SARS-CoV-2 in the aquatic environment, we present the zebrafish as an animal model for translational COVID-19 research
Keywords
CoronavirusDanio rerio
Environmental impacts
Infection diseases
Acute respiratory syndrome
Publisher
Elsevier
Citation
FERNANDES, Bianca H. V. et al. Toxicity of spike fragments SARS-CoV-2 S protein for zebrafish: A tool to study its hazardous for human health? Science of The Total Environment, v. 813, 152345, p. 1-13, 2022.DOI
10.1016/j.scitotenv.2021.152345ISSN
0048-9697Notes
Renata J. Medeiros. Oswaldo Cruz Foundation. National Institute for Quality Control in Health. Departament of Pharmacology and Toxicology. Laboratory of Physiology, Zebrafish Facility. Rio de Janeiro, RJ, Brasil.Fausto K. Ferraris. Oswaldo Cruz Foundation. Institute Oswaldo Cruz. Department of Pharmacology and Toxicology. Rio de Janeiro, RJ, Brazil.
Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
a Laboratório de Controle Genético e Sanitário, Diretoria Técnica de Apoio ao Ensino e Pesquisa, Faculdade de Medicina da Universidade de São Paulo, Brazil b Laboratório Integrado de Biociências Translacionais (LIBT), Instituto de Biodiversidade e Sustentabilidade (NUPEM), Universidade Federal do Rio de Janeiro (UFRJ), Macaé, RJ, Brazil c Department of Microbiology, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, Brazil d Department of Cell Biology, Institute of Biomedical Sciences, University of São Paulo, Brazil e Reproductive and Molecular Biology Group, Department of Morphology, Institute of Biosciences, Sao Paulo State University, Botucatu, São Paulo, Brazil f Department of Structural and Functional Biology, Institute of Biosciences of Botucatu, São Paulo State University, SP, Brazil g Department of Preventive Veterinary Medicine, São Paulo State University (UNESP), Jaboticabal, Brazil h Department of Pharmacology, Center of Research in Inflammatory Diseases, Ribeirao Preto Medical School, University of São Paulo, Brazil i Brasil University, Descalvado, São Paulo, Brazil j Universidade Estadual Paulista Júlio de Mesquita Filho, Instituto de Biociências - Departamento de Fisiologia, São Paulo, Brazil k Universidade Estadual Paulista Júlio de Mesquita Filho, São Paulo, Brazil l Postgraduate Program in Health Sciences, PROCISA, Federal University of Roraima, Brazil m Immunochemistry Laboratory, Butantan Institute, São Paulo, Brazil n Aquaculture Health Research and Extension Group, GRUPESA, Aquaculture Health Laboratory, LABSA, Department of Veterinary Medicine, Federal University of Rondônia, Rolim de Moura campus, Rondônia, Brazil o Department of Preventive Veterinary Medicine, São Paulo State University, Jaboticabal, Brazil p Department of Cell and Molecular Biology, Ribeirao Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil q Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Brazil r Pontifícia Universidade Católica de Minas Gerais, Brazil s Departamento de Ciências Biomoleculares, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil t Department of Pharmacology and Toxicology, Oswaldo Cruz Foundation, FIOCRUZ, Rio de Janeiro, Brazil u Department of Biochemistry and Immunology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Brazil v Laboratory of Physiology, INCQS/Fiocruz Zebrafish Facility, Departament of Pharmacology and Toxicology, National Institute for Quality Control in Health, Brasil w Transplantation Immunobiology Lab, Department of Immunology, Institute of Biomedical Sciences, Universidade de Sao Paulo, Brazil x Center for Translational Research in Oncology, Cancer Institute of the State of Sao Paulo, Faculty of Medicine, University of São Paulo, Sao Paulo, Brazil y Laboratory of Peptide Biochemistry, Federal University of São Paulo, Brazil z Laboratory of Human Immunology, Department Immunology, Institute Biomedical Sciences, University São Paulo, Sao Paulo, Brazil aa Department of Pharmacology, Universidade Federal de Minas Gerais, Brazil ab Graduate Program of Pharmacology, Federal University of Santa Maria, Brazil ac Laboratory of Fish Physiology, Graduate Program of Bioexperimentation and of Environmental Sciences, University of Passo Fundo, Brazil.
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