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PHENOTYPIC TRAITS OF INDIVIDUALS IN A LONG-TERM COLONY OF ANOPHELES (NYSSORHYNCHUS) AQUASALIS (DIPTERA: CULICIDAE) SHOW VARIABLE SUSCEPTIBILITY TO PLASMODIUM AND SUGGEST CRYPTIC SPECIATION
Rodrigues, Nilton Barnabé et al. | Data do documento: 2021
Autor(es)
Rodrigues, Nilton Barnabé
Villegas, Luís Eduardo Martinez
Duarte, Ana Paula Marques
Orfanó, Alessandra Silva
Costa, Breno Dos Anjos
Pimenta, Rafael Nacif
Lacerda, Marcus Vinícius Guimarães de
Monteiro, Wuelton Marcelo
Secundino, Nágila Francinete Costa
Pimenta, Paulo Filemon Paolucci
Afiliação
Laboratory of Medical Entomology. Institute René Rachou. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil.
Laboratory of Medical Entomology. Institute René Rachou. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil.
Institute of Clinical Research Borborema. Tropical Medicine Foundation Dr. Heitor Vieira Dourado. Manaus, AM, Brazi/ Graduation Program in Tropical Medicine. Amazonas State University. Manaus, AM, Brazil/Foundation of Tropical Medicine Dr. Heitor Vieira Dourado. Manaus, AM, Brazil.
Laboratory of Medical Entomology. Institute René Rachou. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil.
Laboratory of Medical Entomology. Institute René Rachou. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil.
Laboratory of Medical Entomology. Institute René Rachou. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil.
Institute of Clinical Research Borborema. Tropical Medicine Foundation Dr. Heitor Vieira Dourado. Manaus, AM, Brazil/Graduation Program in Tropical Medicine. Amazonas State University. Manaus, AM, Brazil/Foundation of Tropical Medicine Dr. Heitor Vieira Dourado. Manaus, AM, Brazil/Institute Leônidas and Maria Deane. Oswaldo Cruz Foundation. Manaus, AM, Brazil.
Institute of Clinical Research Borborema. Tropical Medicine Foundation Dr. Heitor Vieira Dourado. Manaus, AM, Brazi/ Graduation Program in Tropical Medicine. Amazonas State University. Manaus, AM, Brazil/Foundation of Tropical Medicine Dr. Heitor Vieira Dourado. Manaus, AM, Brazil.
Laboratory of Medical Entomology. Institute René Rachou. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil/ Graduation Program in Tropical Medicine. Amazonas State University. Manaus, AM, Brazil.
Laboratory of Medical Entomology. Institute René Rachou. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil/ Institute of Clinical Research Borborema. Tropical Medicine Foundation Dr. Heitor Vieira Dourado. Manaus, AM, Brazil/Graduation Program in Tropical Medicine. Amazonas State University. Manaus, AM, Brazil/ Foundation of Tropical Medicine Dr. Heitor Vieira Dourado. Manaus, AM, Brazil/Institute Leônidas and Maria Deane. Oswaldo Cruz Foundation. Manaus, AM, Brazil.
Resumo em Inglês
Anopheles aquasalis is an important malaria vector in coastal regions of South America and islands of the Caribbean. In its original description, the species was divided into two varieties, based on the scaling patterns of their hind-tarsomere 2. Specimens from our 25-year established colony, used for Plasmodium experimental infections, still exhibit both scaling tarsomere patterns. This study examined the DNA sequence of the nuclear Internal Transcribed Spacer 2 (ITS2) and susceptibility to Plasmodium, looking for differences among the phenotypes 30BS and 50BS. One hundred mosquitoes, 25 males and 25 females of each sex, and phenotype were analyzed. Twenty-seven novel haplotypes were identified. Three were found in both phenotypes (30BS and 50BS) regardless of gender. Among the other 27 genotypes, we observed a male-oriented bias in both phenotypic categories. Evaluation of Plasmodium yoelii N67 infections, based on oocyst counts, showed a higher susceptibility of 30BS compared with 50BS. Future studies need to be conducted to evaluate if these genotype assortments among the phenotypic groups reflect differences in fitness, mating, and their susceptibility to infection by Plasmodium parasites.
Palavras-chave em inglês
Anopheles aquasalis
Genetic variability
rDNA Internal Transcribed Spacer 2 (ITS2)
 
Editor
Elsevier
Referência
RODRIGUES,, Nilton Barnabé et al. Phenotypic traits of individuals in a long-term colony of Anopheles (Nyssorhynchus) aquasalis (Diptera: Culicidae) show variable susceptibility to Plasmodium and suggest cryptic speciation. Acta Trop., v. 224, 106129, 2021. doi: 10.1016/j.actatropica.2021.106129.
DOI
10.1016/j.actatropica.2021.106129
ISSN
0001-706X