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H2B.V DEMARCATES DIVERGENT STRAND-SWITCH REGIONS, SOME TDNA LOCI, AND GENOME COMPARTMENTS IN TRYPANOSOMA CRUZI AND AFFECTS PARASITE DIFFERENTIATION AND HOST CELL INVASION
High Mobility Group Proteins
Cell Differentiation
Host-Parasite Interactions
Proteínas del Grupo de Alta Movilidad
Diferenciación Celular
Interacciones Huésped-Parásitos
Proteínas de Grupo de Alta Mobilidade
Interações Hospedeiro-Parasita
Diferenciação Celular
Autor
Afiliación
Resumen en ingles
Histone variants play a crucial role in chromatin structure organization and gene expression. Trypanosomatids have an unusual H2B variant (H2B.V) that is known to dimerize with the variant H2A.Z generating unstable nucleosomes. Previously, we found that H2B.V protein is enriched in tissue-derived trypomastigote (TCT) life forms, a nonreplicative stage of Trypanosoma cruzi, suggesting that this variant may contribute to the differences in chromatin structure and global transcription rates observed among parasite life forms. Here, we performed the first genome-wide profiling of histone localization in T. cruzi using epimastigotes and TCT life forms, and we found that H2B.V was preferentially located at the edges of divergent transcriptional strand switch regions, which encompass putative transcriptional start regions; at some tDNA loci; and between the conserved and disrupted genome compartments, mainly at trans-sialidase, mucin and MASP genes. Remarkably, the chromatin of TCT forms was depleted of H2B.V-enriched peaks in comparison to epimastigote forms. Interactome assays indicated that H2B.V associated specifically with H2A.Z, bromodomain factor 2, nucleolar proteins and a histone chaperone, among others. Parasites expressing reduced H2B.V levels were associated with higher rates of parasite differentiation and mammalian cell infectivity. Taken together, H2B.V demarcates critical genomic regions and associates with regulatory chromatin proteins, suggesting a scenario wherein local chromatin structures associated with parasite differentiation and invasion are regulated during the parasite life cycle.
Palabras clave en portugues
Trypanosoma cruziPalabras clave en ingles
HistonesHigh Mobility Group Proteins
Cell Differentiation
Host-Parasite Interactions
Palabras clave
HistonasProteínas del Grupo de Alta Movilidad
Diferenciación Celular
Interacciones Huésped-Parásitos
DeCS
HistonasProteínas de Grupo de Alta Mobilidade
Interações Hospedeiro-Parasita
Diferenciação Celular
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