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Tipo
ArtículoDerechos de autor
Acceso restringido
Fecha del embargo
2023
Objetivos de Desarrollo Sostenible
12 Consumo e produção responsáveisColecciones
- IOC - Artigos de Periódicos [12874]
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Mostrar el registro completo del ítem7
CITATIONS
7
Total citations
5
Recent citations
0.89
Field Citation Ratio
0.66
Relative Citation Ratio
VIROME IN ROOF-HARVESTED RAINWATER OF A DENSELY URBANIZED LOW-INCOME REGION
Autor
Afiliación
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vírus Respiratório e do Sarampo. Rio de Janeiro, RJ, Brasil.
Instituto SENAI de Inovação em Biossintéticos e Fibras, Centro Tecnológico da Indústria Química e Têxtil, SENAI CETIQT. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vírus Respiratório e do Sarampo. Rio de Janeiro, RJ, Brasil.
Instituto SENAI de Inovação em Biossintéticos e Fibras, Centro Tecnológico da Indústria Química e Têxtil, SENAI CETIQT. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
Resumen en ingles
Rainwater harvesting has been considered an affordable practice to supplement the conventional sources of
water supply for potable and non-potable uses worldwide. This study characterizes the viral community found
in roof-harvested rainwater (RHRW) samples obtained under different rain volumes in a densely urbanized
low-income region in Rio de Janeiro, Brazil. Three pilot-scale standardized metal-sheet roofs (same catchment
area, material age, and slope – 3%) were installed in the study area aiming at obtaining more reliable and representative
samples. Fifty-four samples were collected fromsix rainfall events fromJanuary to April 2019 and concentrated
by the skimmed-milk flocculation method. Pools of different rainfall volumes were submitted to high
throughput sequencing using the shotgunmetagenomic approach. Sequencing was performed on NextSeq platform.
Genomic analysis of the virus community revealed that most are RNA non-human viruses, including two
main families: Dicistroviridae and Iflaviridae, recognized for infecting arthropods. Bacteriophages were also relatively
abundant, with a predominance of DNA phages belonging to Microviridae and Siphoviridae families, showing
percentages from5.3 and 3.7% of the total viral hits present in these samples, respectively. Viral genomic RNA
viruses (77%) predominated over DNA viruses (23%). Concerning number of viral species identified, a higher percentage
was observed for plant viruses (12 families, 58%). Hepatitis A virus and human klassevirus 1 were detected
among the established human pathogens, suggesting the need for RHRW treatment before it is considered for human consumption. Australian bat lyssavirus was also detected, emphasizing the importance of
environmentalmonitoring facing emerging viruses. The results corroborate the influence of the surrounding area on the rainwater quality.
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