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IN VITRO ANTIBACTERIAL ACTION ON METHICILLIN-SUSCEPTIBLE (MSSA) AND METHICILLIN-RESISTANT (MRSA) STAPHYLOCOCCUS AUREUS AND ANTITUMOR POTENTIAL OF MAURITIA FLEXUOSA L. F.
antibacterial activity
antitumoral activity
phenolic compounds
Autor(es)
Siqueira Filho, Ezequias Pessoa de
Andrade, Anderson Assunção
Fagundes, Elaine Maria de Souza
Ramos, Jonas Pereira
Kohlhoff, Markus
Nunes, Yule Roberta Ferreira
Veloso, Maria das Dores Magalhães
Campos, Fernanda Fraga
Johann, Susana
Alves, Tânia Maria de Almeida
Zani, Carlos Leomar
Cota, Betania Barros
Andrade, Anderson Assunção
Fagundes, Elaine Maria de Souza
Ramos, Jonas Pereira
Kohlhoff, Markus
Nunes, Yule Roberta Ferreira
Veloso, Maria das Dores Magalhães
Campos, Fernanda Fraga
Johann, Susana
Alves, Tânia Maria de Almeida
Zani, Carlos Leomar
Cota, Betania Barros
Afiliação
Oswaldo Cruz Foundation. René Rachou Research Center. Belo Horizonte, MG, Brazil.
Federal University of Triangulo Mineiro. Laboratory of Microbiology, Institute of Biological and Natural Sciences. Uberaba, MG, Brazil.
Federal University of Minas Gerais. Institute of Biological Sciences. Department of Physiology and Biophysics. Belo Horizonte, MG, Brazil.
Federal University of Minas Gerais. Institute of Biological Sciences. Department of Physiology and Biophysics. Belo Horizonte, MG, Brazil.
Oswaldo Cruz Foundation. René Rachou Research Center. Belo Horizonte, MG, Brazil.
Estadual University of Montes Claros.Department of General Biology. Ecology Laboratory and Plant Propagation. Montes Claros, MG, Brazil.
Estadual University of Montes Claros.Department of General Biology. Ecology Laboratory and Plant Propagation. Montes Claros, MG, Brazil.
Federal University of the Valleys of the Jequitinhonha and Mucuri. Department of Biological Sciences and Health. Diamantina, Brazil.
Department of Microbiology. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil.
Oswaldo Cruz Foundation. René Rachou Research Center. Belo Horizonte, MG, Brazil.
Oswaldo Cruz Foundation. René Rachou Research Center. Belo Horizonte, MG, Brazil.
Oswaldo Cruz Foundation. René Rachou Research Center. Belo Horizonte, MG, Brazil.
Federal University of Triangulo Mineiro. Laboratory of Microbiology, Institute of Biological and Natural Sciences. Uberaba, MG, Brazil.
Federal University of Minas Gerais. Institute of Biological Sciences. Department of Physiology and Biophysics. Belo Horizonte, MG, Brazil.
Federal University of Minas Gerais. Institute of Biological Sciences. Department of Physiology and Biophysics. Belo Horizonte, MG, Brazil.
Oswaldo Cruz Foundation. René Rachou Research Center. Belo Horizonte, MG, Brazil.
Estadual University of Montes Claros.Department of General Biology. Ecology Laboratory and Plant Propagation. Montes Claros, MG, Brazil.
Estadual University of Montes Claros.Department of General Biology. Ecology Laboratory and Plant Propagation. Montes Claros, MG, Brazil.
Federal University of the Valleys of the Jequitinhonha and Mucuri. Department of Biological Sciences and Health. Diamantina, Brazil.
Department of Microbiology. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil.
Oswaldo Cruz Foundation. René Rachou Research Center. Belo Horizonte, MG, Brazil.
Oswaldo Cruz Foundation. René Rachou Research Center. Belo Horizonte, MG, Brazil.
Oswaldo Cruz Foundation. René Rachou Research Center. Belo Horizonte, MG, Brazil.
Resumo em Inglês
Mauritia flexuosa L.f. (Arecaceae), commonly known as the buriti palm, is used in traditional medicine in Brazil. Oil from fruits is used for wound healing of skin burns and as an anti rheumatic agent. The stem pith is used to treat dysentery and diarrhea. The aim of this study was to evaluate the in vitro antibacterial and cytotoxic activities of M. flexuosa L.f. extracts and fractions. Crude ethanol extracts (EtOH) from fruits, leaves, and stems were submitted to liquid-liquid partition to yield four different soluble fractions: hexane (Hex), dichloromethane (DCM), ethyl acetate (EtOAc) and aqueous (Aq). The antibacterial activity was evaluated using the broth micro dilution method against methicillin-susceptible S. aureus (MSSA - ATCC 29213) and methicillin-resistant S. aureus (MRSA – clinical sample 155). Cytotoxicity activity was determined against five human tumor cell lines (HL-60, Jurkat, THP-1, MCF-7, and HCT-116) and Vero cell as a model for toxicity control, using MTT colorimetric assay. Across all extracts and fractions assayed, only DCM fractions from leaves and stems were active against S. aureus (MSSA and MRSA) at concentrations ranging from 31.3 to 62.5 µg/ml, and against tumor cell lines with IC50 values ranging from 20.3 to 79.0 µg/ml. The DCM fraction from stems was purified by gel permeation chromatography (GPC) to yield G1 to G12 groups. Groups G7 to G12 showed antimicrobial activity against MSSA and MRSA with MIC values of 31.3 to 125.0 µg/ml and four groups (G3, G9, G10, G12) exhibited cytotoxic activities in the range of 20 µg/ml against at least one tumor cell line. Using the hyphenated methodology, liquid chromatography-mass spectrometry (LC-MS), it was possible to identify several phenolic compounds in subgroups G7 to G10 that can be associated with the medicinal properties of stems from buriti.
Palavras-chave em inglês
Mauritia flexuosaantibacterial activity
antitumoral activity
phenolic compounds
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