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https://www.arca.fiocruz.br/handle/icict/49732
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ArtigoDireito Autoral
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THE SECRETOME FROM EMBRYONIC STEM CELL CARDIOMYOGENESIS: SAME SIGNALS, DIFFERENT CELLULAR FEEDBACKS
Cell Differentiation
Culture Media Conditioned
Embryonic Stem Cells
Diferenciación Celular
Medios de Cultivo Condicionados
Células Madre Embrionarias
Diferenciação Celular
Meios de Cultivo Condicionados
Células-Tronco Embrionárias
Afiliação
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.
Resumo em Inglês
Ischemic heart diseases are a global health problem that requires the search for alternative therapies to the current treatments. Thus, an understanding of how cardiomyogenic signals can affect cellular behavior would allow us to create strategies to improve the cell recovery in damaged tissues. In this study, we aimed to evaluate the effects of the conditioned medium (CM), collected at different time points during in vitro cardiomyogenesis of human embryonic stem cells (hESCs), to direct cell behavior. We assayed different cell types to demonstrate noncytotoxic effects from the collected CM and that the CM obtained at initial time points of cardiomyogenic differentiation could promote the cell proliferation. Otherwise, the secretome derived from cardiac committed cells during cardiomyogenesis was unable to improve angiogenesis or migration in endothelial cells, and ineffective to stimulate the differentiation of cardioblasts or increase the differentiation efficiency of hESC. Therefore, we demonstrated that the effectiveness of the CM response varies depending on the cell type and the differentiation step of hESC-derived cardiomyocytes.
Palavras-chave
SecretomePalavras-chave em inglês
Myocytes CardiacCell Differentiation
Culture Media Conditioned
Embryonic Stem Cells
Palavras-chave em espanhol
Miocitos CardíacosDiferenciación Celular
Medios de Cultivo Condicionados
Células Madre Embrionarias
DeCS
Miócitos CardíacosDiferenciação Celular
Meios de Cultivo Condicionados
Células-Tronco Embrionárias
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