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GENETIC CHARACTERIZATION OF A NEW HIV-1 SUB-SUBTYPE A IN CABO VERDE, DENOMINATED A8
Author
Affilliation
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil.
Universidade de Cabo Verde. Faculdade de Ciências e Tecnologia. Praia 379C, Cape Verde.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil.
Universidade de Cabo Verde. Faculdade de Ciências e Tecnologia. Praia 379C, Cape Verde.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil.
Abstract
Previous molecular characterization of Human immunodeficiency virus (HIV-1) samples
from Cabo Verde pointed out a vast HIV-1 pol diversity, with several subtypes and recombinant forms,
being 5.2% classified as AU-pol. Thus, the aim of the present study was to improve the characterization
of these AU sequences. The genomic DNA of seven HIV-1 AU pol-infected individuals were submitted
to four overlapping nested-PCR fragments aiming to compose the full-length HIV-1 genome. The final
classification was based on phylogenetic trees that were generated using the maximum likelihood
and bootscan analysis. The genetic distances were calculated using Mega 7.0 software. Complete
genome amplification was possible for two samples, and partial genomes were obtained for the other
five. These two samples grouped together with a high support value, in a separate branch from the
other sub-subtypes A and CRF26_A5U. No recombination was verified at bootscan, leading to the
classification of a new sub-subtype A. The intragroup genetic distance from the new sub-subtype
A at a complete genome was 5.2%, and the intergroup genetic varied from 8.1% to 19.0% in the
analyzed fragments. Our study describes a new HIV-1 sub-subtype A and highlights the importance
of continued molecular surveillance studies, mainly in countries with high HIV molecular diversity.
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