Please use this identifier to cite or link to this item:
BLOCKING IL-10 SIGNALING WITH SOLUBLE IL-10 RECEPTOR RESTORES IN VITRO SPECIFIC LYMPHOPROLIFERATIVE RESPONSE IN DOGS WITH LEISHMANIASIS CAUSED BY LEISHMANIA INFANTUM
Author
Affilliation
Abstract
rIL-10 plays a major role in restricting exaggerated inflammatory and immune responses,
thus preventing tissue damage. However, the restriction of inflammatory and immune
responses by IL-10 can also favor the development and/or persistence of chronic infections
or neoplasms. Dogs that succumb to canine leishmaniasis (CanL) caused by L. infantum
develop exhaustion of T lymphocytes and are unable to mount appropriate cellular immune
responses to control the infection. These animals fail to mount specific lymphoproliferative
responses and produce interferon gamma and TNF-alpha that would activate macrophages
and promote destruction of intracellular parasites. Blocking IL-10 signaling may contribute
to the treatment of CanL. In order to obtain a tool for this blockage, the present work endeavored
to identify the canine casIL-10R1 amino acid sequence, generate a recombinant baculovirus
chromosome encoding this molecule, which was expressed in insect cells and
subsequently purified to obtain rcasIL-10R1. In addition, rcasIL-10R1 was able to bind to
homologous IL-10 and block IL-10 signaling pathway, as well as to promote lymphoproliferation
in dogs with leishmaniasis caused by L. infantum.
Share