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PEQUI (CARYOCAR BRASILIENSE CAMBESS)-LOADED NANOEMULSION, ORALLY DELIVERED, MODULATES INFLAMMATION IN LPS-INDUCED ACUTE LUNG INJURY IN MICE
Nanoemulsão
Entrega de drogas
Ácido oleico
Óleo de Pequi
Nanoemulsion
Drug delivery
Pequi oil
Oleic acid
Author
Affilliation
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.
Universidade Federal de Mato Grosso. Instituto de Ciências da Saúde. Sinop, MT, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio Grande do Sul. Instituto de Química. Departamento de Química Orgânica. Porto Alegre, RS, Brasil / Universidade Federal do Rio Grande do Sul. Escola de Farmácia. Porto Alegre, RS, Brasil.
Universidade Federal do Rio Grande do Sul. Escola de Farmácia. Porto Alegre, RS, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.
Universidade Federal de Mato Grosso. Instituto de Ciências da Saúde. Sinop, MT, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.
Universidade Federal de Mato Grosso. Instituto de Ciências da Saúde. Sinop, MT, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio Grande do Sul. Instituto de Química. Departamento de Química Orgânica. Porto Alegre, RS, Brasil / Universidade Federal do Rio Grande do Sul. Escola de Farmácia. Porto Alegre, RS, Brasil.
Universidade Federal do Rio Grande do Sul. Escola de Farmácia. Porto Alegre, RS, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.
Universidade Federal de Mato Grosso. Instituto de Ciências da Saúde. Sinop, MT, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.
Abstract
Pequi is a Brazilian fruit used in folk medicine for pulmonary diseases treatment, but its
oil presents bioavailability limitations. The use of nanocarriers can overcome this limitation.
We developed nanoemulsions containing pequi oil (pequi-NE) and evaluated their e ects in a
lipopolysaccharide (LPS)-induced lung injury model. Free pequi oil or pequi-NE (20 mg/kg) was
orally administered to A/J mice 16 and 4 h prior to intranasal LPS exposure, and the analyses were
performed 24 h after LPS provocation. The physicochemical results revealed that pequi-NE comprised
particles with mean diameter of 174–223 nm, low polydispersity index (0.11 0.01), zeta potential of
7.13 0.08 mV, and pH of 5.83 0.12. In vivo evaluation showed that free pequi oil pretreatment
reduced the influx of inflammatory cells into bronchoalveolar fluid (BALF), while pequi-NE
completely abolished leukocyte accumulation. Moreover, pequi-NE, but not free pequi oil, reduced
myeloperoxidase (MPO), TNF- , IL-1 , IL-6, MCP-1, and KC levels. Similar anti-inflammatory
e ects were observed when LPS-exposed animals were pre-treated with the nanoemulsion containing
pequi or oleic acid. These results suggest that the use of nanoemulsions as carriers enhances the
anti-inflammatory properties of oleic acid-containing pequi oil. Moreover, pequi’s beneficial e ect is
likely due its high levels of oleic acid.Pequi is a Brazilian fruit used in folk medicine for pulmonary diseases treatment, but its
oil presents bioavailability limitations. The use of nanocarriers can overcome this limitation.
We developed nanoemulsions containing pequi oil (pequi-NE) and evaluated their e ects in a
lipopolysaccharide (LPS)-induced lung injury model. Free pequi oil or pequi-NE (20 mg/kg) was
orally administered to A/J mice 16 and 4 h prior to intranasal LPS exposure, and the analyses were
performed 24 h after LPS provocation. The physicochemical results revealed that pequi-NE comprised
particles with mean diameter of 174–223 nm, low polydispersity index (0.11 0.01), zeta potential of
7.13 0.08 mV, and pH of 5.83 0.12. In vivo evaluation showed that free pequi oil pretreatment
reduced the influx of inflammatory cells into bronchoalveolar fluid (BALF), while pequi-NE
completely abolished leukocyte accumulation. Moreover, pequi-NE, but not free pequi oil, reduced
myeloperoxidase (MPO), TNF- , IL-1 , IL-6, MCP-1, and KC levels. Similar anti-inflammatory
e ects were observed when LPS-exposed animals were pre-treated with the nanoemulsion containing
pequi or oleic acid. These results suggest that the use of nanoemulsions as carriers enhances the
anti-inflammatory properties of oleic acid-containing pequi oil. Moreover, pequi’s beneficial e ect is
likely due its high levels of oleic acid.
Keywords in Portuguese
Lesão pulmonar aguda (ALI)/respiratória agudaNanoemulsão
Entrega de drogas
Ácido oleico
Óleo de Pequi
Keywords
Acute lung injury (ALI)/acute respiratory distress syndrome (ARDS);Nanoemulsion
Drug delivery
Pequi oil
Oleic acid
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