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MYCOBACTERIUM AVIUM RESTRICTION FRAGMENT LENGHT POLYMORPHISM-IS IS1245 AND THE SIMPLE DOUBLE REPETITIVE ELEMENT POLYMERASE CHAIN REACTION TYPING METHOD TO SCREEN GENETIC DIVERSITY IN BRAZILIAN STRAINS
AIDS
Micobactérias não Tuberculosas
Reação em Cadeia da Polimerase
Polimorfismo de Fragmento de Restrição
Mycobacterium avium
MaDRE-polymerase chain reaction
Nontuberculous mycobacteria
AIDS
Autor
Afiliación
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Micobacterioses. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Micobacterioses. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Micobacterioses. Rio de Janeiro, RJ, Brasil.
Resumen en ingles
Simple double repetitive element polymerase chain reaction (MaDRE-PCR) and Pvu II-IS1245 restriction fragment length polymorphism (RFLP) typing methods were used to type 41 Mycobacterium avium isolates obtained
from 14 AIDS inpatients and 10 environment and animals specimens identified among 53 mycobacteria isolated
from 237 food, chicken, and pig. All environmental and animals strains showed orphan patterns by both methods. By
MaDRE-PCR four patients, with multiple isolates, showed different patterns, suggesting polyclonal infection that
was confirmed by RFLP in two of them. This first evaluation of MaDRE-PCR on Brazilian M. avium strains demonstrated that the method seems to be useful as simple and less expensive typing method for screening genetic diversity
in M. avium strains on selected epidemiological studies, although with limitation on analysis identical patterns
except for one band.
Palabras clave en portugues
Mycobacterium aviumAIDS
Micobactérias não Tuberculosas
Reação em Cadeia da Polimerase
Polimorfismo de Fragmento de Restrição
Palabras clave en ingles
IS1254-restriction fragment length polymorphismMycobacterium avium
MaDRE-polymerase chain reaction
Nontuberculous mycobacteria
AIDS
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