Autor(es)
Afiliação
Resumo em Inglês
The short duration of viremia, low blood viral load, inaccessibility to timely specific
diagnostic tests, and cross-reactions with other flaviviruses have hindered laboratory confirmation
of Congenital Zika Syndrome (CZS). This study analyzes the positivity of the plaque reduction
neutralization test (PRNT) in children with clinical or imaging characteristics of CZS and its association
with laboratory, clinical, and imaging characteristics. The 94 clinical cases of CZS submitted to the
ZIKV PRNT90 test were followed from 2016 to 2018. The mean age of children at PRNT90 collection
was 22 6 months Standard Deviation. The ZIKV PRNT90 was positive (titer 10) in 40 (42.5%)
children. ZIKV PRNT90 positivity was associated with severe microcephaly in newborns (p = 0.016), lower head circumference z-score at birth (p = 0.043) and 24 months of age (p = 0.031), and severe
reduction of the cerebral parenchyma volume (p = 0.021), expressing greater disease severity. Negative
PRNT90 in children with characteristic signs of CZS may be due to false-negative results, indicating
that the diagnosis of CZS should be primarily syndromic.
Editor
MDPI
Referência
RIBEIRO, Marizélia R. C. et al. Plaque Reduction Neutralization Test (PRNT) in the Congenital Zika Syndrome: Positivity and Associations with Laboratory, Clinical, and Imaging Characteristics. Viruses, v. 12, 2020.
DOI
10.3390/v12111244
ISSN
1999-4915
Notas
1 Department of Medicine III, Federal University of Maranhão, Praça Gonçalves Dias, 21, Centro,
São Luís CEP 65020-240, MA, Brazil
2 Laboratory of Vector-Borne Infectious Diseases, Gonçalo Moniz Institute, Fiocruz-Bahia, Rua Waldemar
Falcão, 121, Candeal, Salvador CEP 40296-710, BA, Brazil; ricardo_khouri@hotmail.com
3 Department of Pathology and Legal Medicine, Faculty of Medicine, Federal University of Bahia,
Praça XV de novembro, s/n–Largo do Terreiro de Jesus, Salvador CEP 40026-010, BA, Brazil
4 Reference Center on Neurodevelopment, Assistance and Rehabilitation of Children/NINAR– Health
Secretariat of the State of Maranhão, Av. dos Holandeses, s/n, São Luís CEP 65077-357, MA, Brazil;
cdneuropatricia@gmail.com
5 Department of Pathology, Federal University of Maranhão, Praça Madre Deus, 2, Madre Deus,
São Luís CEP 65025-560, MA, Brazil; mrfcbranco@gmail.com
6 Department of Public Health, Federal University of Maranhão, Rua Barão de Itapary, 155, Centro,
São Luís CEP 65020-070, MA, Brazil; rosangela.flb@ufma.br (R.F.L.B.); mtssb.alves@ufma.br (M.T.S.S.B.A.);
anakaroltm@gmail.com (A.K.T.M.); tamiresbarradas@gmail.com (T.B.C.);
luciana13cavalcante@gmail.com (L.C.C.); silva.antonio@ufma.br (A.A.M.S.)
7 Department of Medicine I, Federal University of Maranhão, Praça Gonçalves Dias, 21, Centro,
São Luís CEP 65020-240, MA, Brazil; elaine.pfc68@gmail.com
8 Sarah Network of Neurorehabilitation Hospitals, Av. Luis Rocha, s/n, Monte Castelo,
São Luís CP 65035-270, MA, Brazil; glaucio_amaral@yahoo.com.br (G.A.A.);
elimorioka@gmail.com (E.H.M.T.)
9 Diagnostic Imaging Department, Federal University of São Paulo, Rua Napoleão de Barros, 800,
São Paulo CEP 04024-002, SP, Brazil; mborges@huhsp.org.br
10 Laboratory of Human Immunology, Department of Immunology, Institute of Biomedical Sciences,
University of São Paulo, Av. Prof. Lineu Prestes, 1730, Cidade Universitária,
São Paulo CEP 05508-900, SP, Brazil; lilliangomes20@gmail.com
11 Laboratory of Virology, Institute of Tropical Medicine, University of São Paulo, Av. Dr. Enéas Carvalho de
Aguiar, 470, São Paulo CEP 05403-000, SP, Brazil; clarafelix@usp.br (A.C.F.);
nathalia_santiago21@hotmail.com (N.C.S.S.)
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