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COMPLETE NUCLEOTIDE SEQUENCE ANALYSIS OF A BRAZILIAN DENGUE VIRUS TYPE 2 STRAIN
Author
Affilliation
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.
University of California. School of Public Health. Division of Infectious Diseases. Berkeley, CA, USA.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.
University of California. School of Public Health. Division of Infectious Diseases. Berkeley, CA, USA.
University of California. School of Public Health. Division of Infectious Diseases. Berkeley, CA, USA.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.
University of California. School of Public Health. Division of Infectious Diseases. Berkeley, CA, USA.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.
University of California. School of Public Health. Division of Infectious Diseases. Berkeley, CA, USA.
University of California. School of Public Health. Division of Infectious Diseases. Berkeley, CA, USA.
Abstract
In the last decade, dengue fever (DF) in Brazil has been recognized as an important public health problem, and
an increasing number of dengue haemorrhagic fever (DHF) cases have been reported since the introduction of
dengue virus type 2 (DEN-2) into the country in 1990. In order to analyze the complete genome sequence of a DEN2 Brazilian strain (BR64022/98), we designed primers to amplify contiguous segments of approximately 500 base
pairs across the entire sequence of the viral genome. Twenty fragments amplified by reverse transcriptase-PCR were
cloned, and the complete nucleotide and the deduced amino acid sequences were determined. This constitutes the
first complete genetic characterization of a DEN-2 strain from Brazil. All amino acid changes differentiating strains
related to the Asian/American-Asian genotype were observed in BR64022/98, indicating the Asiatic origin of the
strain.
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