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DETECTION AND CHARACTERIZATION OF ROTAVIRUS G AND P TYPES FROM CHILDREN PARTICIPATING IN A ROTAVIRUS VACCINE TRIAL IN BELÉM, BRAZIL
Affilliation
Fundação Nacional de Saúde. Instituto Evandro Chagas. Belém, PA, Brasil.
Fundação Nacional de Saúde. Instituto Evandro Chagas. Belém, PA, Brasil.
Fundação Nacional de Saúde. Instituto Evandro Chagas. Belém, PA, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.
Fundação Nacional de Saúde. Instituto Evandro Chagas. Belém, PA, Brasil.
Fundação Nacional de Saúde. Instituto Evandro Chagas. Belém, PA, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.
Abstract
This study sought the characterization of rotaviruses in a trial with a tetravalent rhesus-human rotavirus vaccine
in Belém, Brazil in children who received three doses of vaccine or placebo in the 1st, 3rd and 5th months of life.
Rotavirus electropherotypes, subgroups, G serotypes, G, [P] and [P],G genotypes were determined in 93.3%, 95.9%,
93.3%, 73.3%, 95.5% and 92.2% of isolates, respectively. Serotypes G1, G2 and G4 were detected in 58.9%, 30% and
4.4% of the cases, respectively. Rotavirus genotype G5 was detected for the first time in Northern region in 4.4% of the
infections. Rotavirus genotypes P[8], P[4], P[6] and P[8+6] were detected in 54.5%, 26.7%, 12.2%, and 2.2% of the
cases, respectively. The predominant genotypes were P[8],G1 and P[4],G2 with 53% and 26.6% of the infections,
respectively. Unusual strains accounted for 20.5% including P[4],G1, P[6],G1, P[6],G4, P[6],G5, P[8],G2, P[8],G5.
Mixed infections involving P[8+6],G2 and P[8+6],G1 were also noted. The neonatal P[6] strains associated with
diarrhea were detected among children aged 9-24 months. To our knowledge, this study represents the first in Brazil
to analyse, on molecular basis, rotavirus genotypes from children participating in a rotavirus vaccine trial. These
results are of potential importance regarding future rotavirus vaccination strategies in Brazil.
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