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BINDING OF LEISHMANIA INFANTUM LIPOPHOSPHOGLYCAN TO THE MIDGUT IS NOT SUFFICIENT TO DEFINE VECTOR COMPETENCE IN LUTZOMYIA LONGIPALPIS SAND FLIES
Leishmania
Parasite binding
Parasite survival
Sand fly
Sand fly midgut
Vector competence
Leishmania infantum
Autor(es)
Afiliação
National Institutes of Health. National Institute of Allergy and Infectious Diseases. Vector Molecular Biology Section, Laboratory of Malaria and Vector Research. Rockville, Maryland, USA.
National Institutes of Health. National Institute of Allergy and Infectious Diseases. Vector Molecular Biology Section, Laboratory of Malaria and Vector Research. Rockville, Maryland, USA.
National Institutes of Health. National Institute of Allergy and Infectious Diseases. Vector Molecular Biology Section, Laboratory of Malaria and Vector Research. Rockville, Maryland, USA.
National Institutes of Health. National Institute of Allergy and Infectious Diseases. Vector Molecular Biology Section, Laboratory of Malaria and Vector Research. Rockville, Maryland, USA.
National Institutes of Health. National Institute of Allergy and Infectious Diseases. Vector Molecular Biology Section, Laboratory of Malaria and Vector Research. Rockville, Maryland, USA.
Fundação Oswaldo Cruz. Instituto René rachou. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz, Salvador, BA, Brasil.
Centre Armand-Frappier Santé Biotechnologie. Laval, Quebec, Canada.
National Institutes of Health. National Institute of Allergy and Infectious Diseases. Vector Molecular Biology Section, Laboratory of Malaria and Vector Research. Rockville, Maryland, USA.
National Institutes of Health. National Institute of Allergy and Infectious Diseases. Vector Molecular Biology Section, Laboratory of Malaria and Vector Research. Rockville, Maryland, USA.
National Institutes of Health. National Institute of Allergy and Infectious Diseases. Vector Molecular Biology Section, Laboratory of Malaria and Vector Research. Rockville, Maryland, USA.
National Institutes of Health. National Institute of Allergy and Infectious Diseases. Vector Molecular Biology Section, Laboratory of Malaria and Vector Research. Rockville, Maryland, USA.
National Institutes of Health. National Institute of Allergy and Infectious Diseases. Vector Molecular Biology Section, Laboratory of Malaria and Vector Research. Rockville, Maryland, USA.
National Institutes of Health. National Institute of Allergy and Infectious Diseases. Vector Molecular Biology Section, Laboratory of Malaria and Vector Research. Rockville, Maryland, USA.
Fundação Oswaldo Cruz. Instituto René rachou. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz, Salvador, BA, Brasil.
Centre Armand-Frappier Santé Biotechnologie. Laval, Quebec, Canada.
National Institutes of Health. National Institute of Allergy and Infectious Diseases. Vector Molecular Biology Section, Laboratory of Malaria and Vector Research. Rockville, Maryland, USA.
National Institutes of Health. National Institute of Allergy and Infectious Diseases. Vector Molecular Biology Section, Laboratory of Malaria and Vector Research. Rockville, Maryland, USA.
Resumo em Inglês
The major surface lipophosphoglycan (LPG) of Leishmania parasites is
critical to vector competence in restrictive sand fly vectors in mediating Leishmania
attachment to the midgut epithelium, considered essential to parasite survival and
development. However, the relevance of LPG for sand flies that harbor multiple species
of Leishmania remains elusive. We tested binding of Leishmania infantum wildtype
(WT), LPG-defective (Δlpg1 mutants), and add-back (Δlpg1 LPG1) lines to sand
fly midguts in vitro and their survival in Lutzomyia longipalpis sand flies in vivo. Le.
infantum WT parasites attached to the Lu. longipalpis midgut in vitro, with late-stage
parasites binding to midguts in significantly higher numbers than were seen with
early-stage promastigotes. Δlpg1 mutants did not bind to Lu. longipalpis midguts,
and this was rescued in the Δlpg1 LPG1 lines, indicating that midgut binding is
mediated by LPG. When Lu. longipalpis sand flies were infected with the Le. infantum
WT or Le. infantum Δlpg1 or Le. infantum Δlpg1 LPG1 line of the BH46 or BA262
strains, the BH46 Δlpg1 mutant, but not the BA262 Δlpg1 mutant, survived and grew
to numbers similar to those seen with the WT and Δlpg1 LPG1 lines. Exposure of
BH46 and BA262 Δlpg1 mutants to blood-engorged midgut extracts led to mortality
of the BA262 Δlpg1 but not the BH46 Δlpg1 parasites. These findings suggest that
Le. infantum LPG protects parasites on a strain-specific basis early in infection, likely
against toxic components of blood digestion, but that it is not necessary to prevent
Le. infantum evacuation along with the feces in the permissive vector Lu. longipalpis.
Palavras-chave em inglês
LPGLeishmania
Parasite binding
Parasite survival
Sand fly
Sand fly midgut
Vector competence
Leishmania infantum
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