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LEISHMANIA MAJOR: PARASITE INTERACTIONS SUGGESTING SEXUALITY
Leishmania major
Sexualidade
Morfologia
Imunocytoquímica
Microscopia eletrônica de varredura
Leishmania major
Sexuality
Morphology
Immunocytochemistry
Scanning electron microscopy
Afiliación
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultraestrutura e Biologia Celular. Coleção de Tripanosomatídeos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultraestrutura e Biologia Celular. Coleção de Tripanosomatídeos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultraestrutura e Biologia Celular. Coleção de Tripanosomatídeos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultraestrutura e Biologia Celular. Coleção de Tripanosomatídeos. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultraestrutura e Biologia Celular. Coleção de Tripanosomatídeos. Rio de Janeiro, RJ, Brasil.
Resumen en ingles
In five experiments, Leishmania (Leishmania) major (MRHO/SU/59/P-strain) grew poorly when
seeded in FYTS medium supplemented with 15% fetal calf serum, but presented several peculiar pairs of
promastigotes diametrically opposed and attached at their posterior ends (5.8-13.5%). As seen in Giemsa-stained smears, a ring-like line and/or an enlargement, generally occurred at the parasite junction. A close proximity of nuclei, which sometimes were difficult to distinguish from each other, was also
observed at this junction. Several of these pairs appeared to be composed of fused cells in which the
nuclei could be apparently fused, as shown by fluorescence microscopy to detect ß-tubulin and DNA,
and by scanning electron microscopy. Under other culture conditions these pairs were absent or occurred at very low rates (0.2-2.2%). Such pairs differ markedly from longitudinally dividing cells and
resemble those described in two other Leishmania species, as well as in Herpetomonas megaseliae and
Phytomonas davidi, suggesting steps of a putative sexual process.
Palabras clave en portugues
TrypanosomatidaeLeishmania major
Sexualidade
Morfologia
Imunocytoquímica
Microscopia eletrônica de varredura
Palabras clave en ingles
TrypanosomatidaeLeishmania major
Sexuality
Morphology
Immunocytochemistry
Scanning electron microscopy
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