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https://www.arca.fiocruz.br/handle/icict/42796
IN VITRO AND IN VIVO INHIBITION OF HCT116 CELLS BY ESSENTIAL OILS FROM BARK AND LEAVES OF VIROLA SURINAMENSIS (ROL. EX ROTTB.) WARB. (MYRISTICACEAE)
Autor(es)
Anunciação, Talita A. da
Costa, Rafaela G. A.
Lima, Emilly J. S. P. de
Silva, Valdenizia Rodrigues
Santos, Luciano de S.
Soares, Milena Botelho Pereira
Dias, Rosane Borges
Rocha, Clarissa Araújo Gurgel
Costa, Emmanoel Vilaça
Silva, Felipe Moura Araujo da
Koolen, Hector Henrique Ferreira
Bezerra, Daniel Pereira
Costa, Rafaela G. A.
Lima, Emilly J. S. P. de
Silva, Valdenizia Rodrigues
Santos, Luciano de S.
Soares, Milena Botelho Pereira
Dias, Rosane Borges
Rocha, Clarissa Araújo Gurgel
Costa, Emmanoel Vilaça
Silva, Felipe Moura Araujo da
Koolen, Hector Henrique Ferreira
Bezerra, Daniel Pereira
Afiliação
Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Salvador, BA, Brasil.
Amazonas State University. Metabolomics and Mass Spectrometry Research Group. Manaus, AM, Brazil.
Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. Faculty of Dentistry, Department of Clinical Propaedeutics and Integrated Clinical. Salvador, BA, Brazil.
Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. Faculty of Dentistry, Department of Clinical Propaedeutics and Integrated Clinical. Salvador, BA, Brazil.
Federal University of Amazonas. Department of Chemistry. Manaus, AM, Brazil.
Federal University of Amazonas. Department of Chemistry. Manaus, AM, Brazil.
Amazonas State University. Metabolomics and Mass Spectrometry Research Group. Manaus, AM, Brazil.
Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Salvador, BA, Brasi
Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Salvador, BA, Brasil.
Amazonas State University. Metabolomics and Mass Spectrometry Research Group. Manaus, AM, Brazil.
Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. Faculty of Dentistry, Department of Clinical Propaedeutics and Integrated Clinical. Salvador, BA, Brazil.
Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. Faculty of Dentistry, Department of Clinical Propaedeutics and Integrated Clinical. Salvador, BA, Brazil.
Federal University of Amazonas. Department of Chemistry. Manaus, AM, Brazil.
Federal University of Amazonas. Department of Chemistry. Manaus, AM, Brazil.
Amazonas State University. Metabolomics and Mass Spectrometry Research Group. Manaus, AM, Brazil.
Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Salvador, BA, Brasi
Resumo em Inglês
Virola surinamensis (Rol. ex Rottb.) Warb. (Myristicaceae), popularly known in Brazil as "mucuíba", "ucuúba", "ucuúba-branca" or "ucuúba do igapó", is a medicinal plant used to treat a variety of diseases, including infections, inflammatory processes and cancer. Aim of the study: In the present work, we investigated the chemical constituents and the in vitro and in vivo
inhibition of human colon carcinoma HCT116 cells by essential oils obtained from the bark (EOB) and leaves
(EOL) of V. surinamensis.
Materials and methods: EOB and EOL were obtained by hydrodistillation and analyzed via gas chromatography
with flame ionization detection and gas chromatography coupled to mass spectrometry. In vitro cytotoxic activity
was determined in cultured cancer cells HCT116, HepG2, HL-60, B16–F10 and MCF-7 and in a non-cancerous cell
line MRC-5 by the Alamar blue assay after 72 h of treatment. Annexin V/propidium iodide staining, mitochondrial
transmembrane potential and cell cycle distribution were evaluated by flow cytometry in HCT116 cells
treated with essential oils after 24 and 48 h of treatment. The cells were also stained with May-Grunwald-Giemsa
to analyze cell morphology. In vivo antitumor activity was evaluated in C.B-17 SCID mice with HCT116 cells.
Results: The main constituents in EOB were aristolene (28.0 � 3.1%), α-gurjunene (15.1 � 2.4%), valencene
(14.1 � 1.9%), germacrene D (7.5 � 0.9%), δ-guaiene (6.8 � 1.0%) and β-elemene (5.4 � 0.6%). On the other
hand, EOL displayed α-farnesene (14.5 � 1.5%), β-elemene (9.6 � 2.3%), bicyclogermacrene (8.1 � 2.0%),
germacrene D (7.4 � 0.7%) and α-cubebene (5.6 � 1.1%) as main constituents. EOB showed IC50 values for
cancer cells ranging from 9.41 to 29.52 μg/mL for HCT116 and B16–F10, while EOL showed IC50 values for
cancer cells ranging from 7.07 to 26.70 μg/mL for HepG2 and HCT116, respectively. The IC50 value for a noncancerous
MRC-5 cell was 34.7 and 38.93 μg/mL for EOB and EOL, respectively. Both oils induced apoptotic-like
cell death in HCT116 cells, as observed by the morphological characteristics of apoptosis, externalization of
phosphatidylserine, mitochondrial depolarization and fragmentation of internucleosomal DNA. At a dose of 40
mg/kg, tumor mass inhibition rates were 57.9 and 44.8% in animals treated with EOB and EOL, respectively.
Conclusions: These data indicate V. surinamensis as possible herbal medicine in the treatment of colon cancer
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