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HUMAN BOCAVIRUS GENOTYPES 1 AND 2 DETECTED IN YOUNGER AMAZONIAN CHILDREN WITH ACUTE GASTROENTERITIS OR RESPIRATORY INFECTIONS, RESPECTIVELY
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Abstract
This study aimed to verify the frequency, genotypes, and etiological role of Human Bocavirus
(HBoV) in younger Amazonian children with either acute gastroenteritis (AGE) or respiratory infections
(ARI). The influence of RotarixTM vaccination and co-infection status was also investigated.
Design: HBoV quantitative polymerase chain reaction (qPCR) testing was done on both fecal and saliva
(1468 samples) from 734 children < 5 months old living in the Amazon (Brazil, Guyana, and Venezuela).
High and median HBoV viral load samples were used for extraction, nested PCR amplification, and
sequencing for genotyping. HBoV mRNA detection was done by reverse transcription following DNA
amplification.
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