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COMPARSION OF FLOW CYTOMETRIC AND ALAMAR BLUE TESTS WITH THE PROPORTIONAL METHOD FOR TESTING SUSCEPTIBILITY OF MYCOBACTERIUM TUBERCULOSIS TO RIFAMPIN AND IZONIAZID
Reis, Roberto S. et al. | Date Issued: 2004
Author
Reis, Roberto S.
Neves Jr, Ivan
Lourenço, Sérgio L. S.
Fonseca, Leila S.
Lourenço, Maria Cristina S.
Affilliation
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Microbiologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.
Abstract
The performance of flow cytometry and the microplate Alamar Blue assay in determining susceptibility of Mycobacterium tuberculosis was assessed by testing 150 Brazilian isolates. The overall agreement was 97.3 and 98% for isoniazid and 94.7 and 100% for rifampin by flow cytometry and MABA, respectively. This study was entirely done in a developing country.
Keywords
Antitubercular Agents
Isoniazid
Microbial Sensitivity Tests
Mycobacterium tuberculosis
Oxazines
Rifampin
Xanthenes
 
Publisher
American Society for Microbiology
Citation
REIS, Roberto S. et al. Comparison of flow cytometric and Alamar Blue tests with the proportional method for testing susceptibility of Mycobacterium tuberculosis to rifampin and isoniazid. Journal of Clinical Microbiology, v. 42, n. 5, p. 2247-2248, 2004.
DOI
10.1128/jcm.42.5.2247-2248.2004
ISSN
0095-1137