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ESSENTIAL OIL FROM LEAVES OF CONOBEA SCOPARIOIDES (CHAM. & SCHLTDL.) BENTH. (PLANTAGINACEAE) CAUSES CELL DEATH IN HEPG2 CELLS AND INHIBITS TUMOR DEVELOPMENT IN A XENOGRAFT MODEL
Autor
Lima, Emilly J. S. P. de
Fontes, Sheila S.
Nogueira, Mateus Lima
Silva, Valdenizia Rodrigues
Santos, Luciano de S.
D'Elia, Gigliola M. A.
Dias, Rosane Borges
Sales, Caroline Brandi Schlaepfer
Rocha, Clarissa Araújo Gurgel
Santos, Marcos André Vannier
Soares, Milena Botelho Pereira
Costa, Emmanoel Vilaça
Silva, Felipe Moura Araujo da
Koolen, Hector Henrique Ferreira
Bezerra, Daniel Pereira
Fontes, Sheila S.
Nogueira, Mateus Lima
Silva, Valdenizia Rodrigues
Santos, Luciano de S.
D'Elia, Gigliola M. A.
Dias, Rosane Borges
Sales, Caroline Brandi Schlaepfer
Rocha, Clarissa Araújo Gurgel
Santos, Marcos André Vannier
Soares, Milena Botelho Pereira
Costa, Emmanoel Vilaça
Silva, Felipe Moura Araujo da
Koolen, Hector Henrique Ferreira
Bezerra, Daniel Pereira
Afiliación
Amazonas State University. Metabolomics and Mass Spectrometry Research Group. Manaus, AM, Brazil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
Amazonas State University. Metabolomics and Mass Spectrometry Research Group. Manaus, AM, Brazil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. Faculty of Dentistry. Department of Clinical Propaedeutics and Integrated Clinical. Salvador, BA, Brazil.
Federal University of Bahia. Institute of Health Sciences. Department of Biomorphology. Salvador, BA, Brazil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. Faculty of Dentistry. Department of Clinical Propaedeutics and Integrated Clinical. Salvador, BA, Brazil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
Federal University of Amazonas. Department of Chemistry. Manaus, AM, Brazil.
Federal University of Amazonas. Department of Chemistry. Manaus, AM, Brazil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
Amazonas State University. Metabolomics and Mass Spectrometry Research Group. Manaus, AM, Brazil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. Faculty of Dentistry. Department of Clinical Propaedeutics and Integrated Clinical. Salvador, BA, Brazil.
Federal University of Bahia. Institute of Health Sciences. Department of Biomorphology. Salvador, BA, Brazil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. Faculty of Dentistry. Department of Clinical Propaedeutics and Integrated Clinical. Salvador, BA, Brazil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
Federal University of Amazonas. Department of Chemistry. Manaus, AM, Brazil.
Federal University of Amazonas. Department of Chemistry. Manaus, AM, Brazil.
Resumen en ingles
Conobea scoparioides (Cham. & Schltdl.) Benth. (syn. Sphaerotheca scoparioides Cham. & Schldtl.) (Plantaginaceae), popularly known as "pataqueira", "vassourinha-do-brejo" and/or "hierba-de-sapo", is a popular medicinal plant used to treat leishmaniasis, pain and beriberi. In addition, inhibition of cell adhesion, antioxidant, cytotoxic and leishmanicidal activities of compounds or fractions of C. scoparioides have been reported. In the present work, chemical constituents and in vitro and in vivo anti-liver cancer potential of essential oil (EO) from leaves of C. scoparioides were investigated using human hepatocellular carcinoma HepG2 cells as a cell model. EO was obtained by hydrodistillation using a Clevenger-type apparatus and characterized by GC-MS and GC-FID. The in vitro cytotoxic effect was evaluated on three human cancer cell lines (MCF-7, HepG2 and HCT116) and one human non-cancerous cell line (MRC-5) using the Alamar blue assay. Phosphatidylserine externalization and cell cycle distribution were quantified in HepG2 cells by flow cytometry after 48 h incubation. The effectiveness of EO in anti-liver cancer model was studied with HepG2 cells grafted on C.B. 17 SCID mice. The main constituents of EO were thymol methyl ether (62 %), thymol (16 %) and α-phellandrene (14 %). EO displayed an in vitro cytotoxic effect against all human cancer cell lines and caused externalization of phosphatidylserine and DNA fragmentation in HepG2 cells, suggesting induction of apoptotic-like cell death. In vivo tumor mass inhibition of 36.7 and 55.8 % was observed for treatment with EO at doses of 40 and 80 mg/kg, respectively. These results indicate in vitro and in vivo anti-liver cancer potential of EO from leaves of C. scoparioides.
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