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ArtigoDireito Autoral
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Objetivos de Desenvolvimento Sustentável
03 Saúde e Bem-EstarColeções
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YELLOW FEVER VIRUS GENOTYPING TOOL AND INVESTIGATION OF SUSPECTED ADVERSE EVENTS FOLLOWING YELLOW FEVER VACCINATION
YFV-17DD
adverse event
arbovirus
yellow fever
vaccination
immunisation
vaccine
genotyping
wild-type virus
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Resumo em Inglês
The yellow fever (YF) vaccine consists of an attenuated virus, and despite its relative
safety, some adverse events following YF vaccination have been described. At the end of 2016, Brazil experienced the most massive sylvatic yellow fever outbreak over the last 70 years and an intense campaign of YF vaccination occurred in Minas Gerais state in Southeast Brazil from 2016 to 2018. The present study aimed to develop a genotyping tool and investigate 21 cases of suspected adverse events following YF vaccination. Initial in silico analyses were performed using partial NS5 nucleotide sequences to verify the discriminatory potential between wild-type and vaccine viruses. Samples from patients were screened for the presence of the YFV RNA, using 50UTR as the target, and then used for amplification of partial NS5 gene amplification, sequencing, and phylogenetic analysis. Genotyping indicated that 17 suspected cases were infected by the wild-type yellow fever virus, but four cases remained inconclusive. The genotyping tool was e_cient in distinguishing the vaccine from wild-type virus, and it has the potential to be used for the di_erentiation of all yellow fever virus genotypes.
Palavras-chave em inglês
yellow fever virusYFV-17DD
adverse event
arbovirus
yellow fever
vaccination
immunisation
vaccine
genotyping
wild-type virus
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