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https://www.arca.fiocruz.br/handle/icict/41328
DDX6 HELICASE BEHAVIOR AND PROTEIN PARTNERS IN HUMAN ADIPOSE TISSUE-DERIVED STEM CELLS DURING EARLY ADIPOGENESIS AND OSTEOGENESIS
Proteínas de Unión al ARN
Adipogénesis
Osteogénesis
Autor(es)
Afiliação
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.
Pontifícia Universidade Católica do Paraná. Faculdade de Medicina. Núcleo de Tecnologia Celular. Curitiba, PR, Brasil.
Universidade Federal do Alagoas. Laboratório de Inovação Farmacológica. Maceió, AL, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.
Pontifícia Universidade Católica do Paraná. Faculdade de Medicina. Núcleo de Tecnologia Celular. Curitiba, PR, Brasil.
Universidade Federal do Alagoas. Laboratório de Inovação Farmacológica. Maceió, AL, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.
Resumo em Inglês
DDX6 helicase is an RNA-binding protein involved in different aspects of gene expression regulation. The roles played by DDX6 depend on the complexes associated with it. Here, for the first time, we characterize the protein complexes associated with DDX6 in human adipose tissue-derived stem cells (hASCs) and analyze the dynamics of this helicase under different conditions of translational activity and differentiation. The results obtained demonstrated that the DDX6 helicase is associated with proteins involved in the control of mRNA localization, translation and metabolism in hASCs. DDX6 complexes may also assemble into more complex structures, such as RNA-dependent granules, the abundance and composition of which change upon inhibited translational activity. This finding supports the supposition that DDX6 is possibly involved in the regulation of the mRNA life cycle in hASCs. Although there was no significant variation in the protein composition of these complexes during early adipogenic or osteogenic induction, there was a change in the distribution pattern of DDX6: the number of DDX6 granules per cell was reduced during adipogenesis and was enhanced during osteogenesis.
Palavras-chave
DDX6 helicasePalavras-chave em espanhol
Células Madre MesenquimatosasProteínas de Unión al ARN
Adipogénesis
Osteogénesis
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