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IMPROVING THE SERODIAGNOSIS OF CANINE LEISHMANIA INFANTUM INFECTION IN GEOGRAPHICAL AREAS OF BRAZIL WITH DIFFERENT DISEASE PREVALENCE
Ramírez, Laura et al. | Fecha del documento: 2020
Autor
Ramírez, Laura
Moura, Luana Dias de
Mateus, Natalia Lopes Fontoura
Moraes, Milene Hoehr de
Nascimento, Leopoldo Fabrício Marçal do
Melo, Nailson de Jesus
Taketa, Lucas Bezerra
Catecati, Tatiana
Huete, Samuel G.
Penichet, Karla
Piranda, Eliane Mattos
Oliveira, Alessandra Gutierrez de
Steindel, Mario
Barral Netto, Manoel
Cruz, Maria do Socorro Pires e
Barral, Aldina Maria Prado
Soto, Manuel
Afiliación
Universidad Autónoma de Madrid. Facultad de Ciencias. Centro de Biología Molecular Severo Ochoa. Departamento de Biología Molecular. Madrid, Spain.
Universidade Federal do Piaui. Centro de Ciências Agrárias. Teresina, PI, Brasil.
Universidade Federal de Mato Grosso do Sul. Centro de Ciências Biológicas e da Saúde. Laboratório de Parasitologia. Campo Grande, MS, Brasil.
Universidade Federal de Santa Catarina. Departamento de Microbiologia, Imunologia e Parasitologia. Florianópolis, SC, Brasil.
Universidade Federal do Piaui. Centro de Ciências Agrárias. Teresina, PI, Brasil.
Universidade Federal do Piaui. Centro de Ciências Agrárias. Teresina, PI, Brasil.
Universidade Federal de Mato Grosso do Sul. Centro de Ciências Biológicas e da Saúde. Laboratório de Parasitologia. Campo Grande, MS, Brasil.
Universidade Federal de Santa Catarina. Departamento de Microbiologia, Imunologia e Parasitologia. Florianópolis, SC, Brasil.
Universidad Autónoma de Madrid. Facultad de Ciencias. Centro de Biología Molecular Severo Ochoa. Departamento de Biología Molecular. Madrid, Spain.
Universidad Autónoma de Madrid. Facultad de Ciencias. Centro de Biología Molecular Severo Ochoa. Departamento de Biología Molecular. Madrid, Spain.
Universidade Federal de Mato Grosso do Sul. Centro de Ciências Biológicas e da Saúde. Laboratório de Parasitologia. Campo Grande, MS, Brasil.
Universidade Federal de Mato Grosso do Sul. Centro de Ciências Biológicas e da Saúde. Laboratório de Parasitologia. Campo Grande, MS, Brasil.
Universidade Federal de Santa Catarina. Departamento de Microbiologia, Imunologia e Parasitologia. Florianópolis, SC, Brasil.
Fundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
Universidade Federal do Piaui. Centro de Ciências Agrárias. Teresina, PI, Brasil.
Fundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
Universidad Autónoma de Madrid. Facultad de Ciencias. Centro de Biología Molecular Severo Ochoa. Departamento de Biología Molecular. Madrid, Spain.
Resumen en ingles
Serodiagnosis of Leishmania infantum infection in dogs relies on the detection of antibodies against leishmanial crude extracts or parasitic defined antigens. The expansion of canine leishmaniasis from geographical areas of Brazil in which the infection is endemic to regions in which the disease is emerging is occurring. This fact makes necessary the analysis of the serodiagnostic capabilities of different leishmanial preparations in distinct geographical locations. In this article sera from dogs infected with Leishmania and showing the clinical form of the disease, were collected in three distinct Brazilian States and were tested against soluble leishmanial antigens or seven parasite individual antigens produced as recombinant proteins. We show that the recognition of soluble leishmanial antigens by sera from these animals was influenced by the geographical location of the infected dogs. Efficacy of the diagnosis based on this crude parasite preparation was higher in newly endemic regions when compared with areas of high disease endemicity. We also show that the use of three of the recombinant proteins, namely parasite surface kinetoplastid membrane protein of 11 kDa (KMP-11), and two members of the P protein family (P2a and P0), can improve the degree of sensitivity without adversely affecting the specificity of the diagnostic assays for canine leishmaniasis, independently of the geographical area of residence. In addition, sera from dogs clinically healthy but infected were also assayed with some of the antigen preparations. We demonstrate that the use of these proteins can help to the serodiagnosis of Leishmania infected animals with subclinical infections. Finally, we propose a diagnostic protocol using a combination of KMP-11, P2a y P0, together with total leishmanial extracts.
Palabras clave en portugues
Leishmania
Leishmaniose canina
Sorodiagnóstico
Anticorpos
Proteínas recombinantes
 
Palabras clave en ingles
Leishmania
Canine leishmaniasis
Serodiagnosis
Antibodies
Recombinant proteins
 
Editor
Elsevier
Referencia
RAMÍREZ, Laura et al. Improving the serodiagnosis of canine Leishmania infantum infection in geographical areas of Brazil with different disease prevalence. Parasite Epidemiology and Control, v. 8, p. 1-12, 2020.
DOI
10.1016/j.parepi.2019.e00126
ISSN
2405-6731