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NEW FINDING OF GIARDIA INTESTINALIS (EUKARYOTE, METAMONAD) IN OLD WORLD ARCHAEOLOGICAL SITE USING IMMUNOFLUORESCENCE AND ENZYME-LINKED IMMUNOSORBENT ASSAYS
Ensaio de imunoadsorção enzimática
Dietilpropiona
Giardia lamblia
Author
Affilliation
Université de Reims. Reims, France / Muséum National d'Histoire Naturelle. Paris, France.
Muséum National d'Histoire Naturelle. Paris, France / Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro, RJ, Brasil.
Université de Reims. Reims, France.
Institut National de Recherches Archéologiques Préventives. Paris, France.
Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro, RJ, Brasil.
Université de Reims. Reims, France.
Muséum National d'Histoire Naturelle. Paris, France / Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro, RJ, Brasil.
Université de Reims. Reims, France.
Institut National de Recherches Archéologiques Préventives. Paris, France.
Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro, RJ, Brasil.
Université de Reims. Reims, France.
Abstract
In this study, nine organic sediment samples from a medieval archaeological site at Pineuilh, France, were examined for Giardia intestinalis using two commercially available immunological kits [enzyme-linked immuno sorbent and immunofluorescence (IFA) assays]. Both techniques detected G. intestinalis in one sample, dated to 1,000 Anno Domini. This is the first time IFA was successfully used to detect protozoa in Old World archaeological samples. Such immunological techniques offer important perspectives concerning ancient protozoa detection and identification.
Keywords in Portuguese
PaleoparasitologiaEnsaio de imunoadsorção enzimática
Dietilpropiona
Giardia lamblia
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