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GENOMIC CHARACTERIZATION OF A BRAZILIAN TT VIRUS ISOLATE CLOSELY RELATED TO SEN VIRUS-F
Vírus da imunodeficiência humana tipo 1
Sequenciamento de nucleotídeos
Torque teno virus
Human immunodeficiency virus type 1
Nucleotide sequencing
Open reading frames 1-4
SEN virus-F
TT virus
Afiliación
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.
Resumen en ingles
SEN virus (SENV) is a circular, single stranded DNA virus that has been first characterized in the serum of a human immunodeficiency virus type 1 (HIV-1)-infected patient. Eight genotypes of SENV (A-H) have been identified and further recognized as variants of TT virus (TTV) in the family Circoviridae. Here we describe the first genomic characterization of a SENV isolate (5-A) from South America. Using ‘universal’ primers, able to amplify most, if not all, TTV/SENV genotypes, a segment of > 3 kb was amplified by polymerase chain reaction from the serum of an HIV-1 infected patient. The amplicon was cloned and a 3087-nucleotide sequence was determined, that showed a high (85%) homology with the sequence of the Italian isolate SENV-F. Proteins encoded by open reading frames (ORFs) 1 to 4 consisted of 758, 129, 276, and 267 amino acids, respectively. By phylogenetic analysis, isolate 5-A was classified into TTV genotype 19 (phylogenetic group 3), together with SENV-F and TTV isolate SAa-38.
Palabras clave en portugues
HepatiteVírus da imunodeficiência humana tipo 1
Sequenciamento de nucleotídeos
Torque teno virus
Palabras clave en ingles
HepatitisHuman immunodeficiency virus type 1
Nucleotide sequencing
Open reading frames 1-4
SEN virus-F
TT virus
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