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https://www.arca.fiocruz.br/handle/icict/36632
Tipo de documento
ArtigoDireito Autoral
Acesso aberto
Data de embargo
2020-10-22
Coleções
- INI - Artigos de Periódicos [3460]
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IDENTIFICATION OF NOVEL HYBRIDS BETWEEN CRYPTOCOCCUS NEOFORMANS VAR. GRUBII VNI AND CRYPTOCOCCUS GATTII VGII
Cryptococcus gattii
AB hybrids
Serotype
Mating type
Molecular type
Autor(es)
Afiliação
Molecular Mycology Research Laboratory. Sydney, NSW, Australia / University of Sydney. Sydney Medical School-Westmead. Westmead Millennium Institute. Centre for Infectious Diseases and Microbiology / University of Sydney. Sydney Emerging Infections and Biosecurity Institute. Sydney, NSW, Australia.
University of Miami. Rosenstiel School of Marine and Atmospheric Sciences. Miami, FL, USA.
National and Kapodistrian University of Athens. Medical School. Department of Microbiology. Mycology Laboratory. Athens, Greece.
Instituto Nacional de Salud. Grupo de Microbiología. Bogotá, Colombia.
Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.
National and Kapodistrian University of Athens. Medical School. Department of Microbiology. Mycology Laboratory. Athens, Greece.
St. Vincent’s Hospital. Department of Microbiology and Clinical Infectious Diseases. Sydney, NSW, Australia.
University of Sydney. Sydney Medical School-Westmead. Westmead Millennium Institute. Centre for Infectious Diseases and Microbiology / University of Sydney. Sydney Emerging Infections and Biosecurity Institute. Sydney, NSW, Australia.
University of Sydney. Sydney Medical School-Westmead. Westmead Millennium Institute. Centre for Infectious Diseases and Microbiology / University of Sydney. Sydney Emerging Infections and Biosecurity Institute. Sydney, NSW, Australia / Westmead Hospital. Molecular Mycology Research Laboratory. Sydney, NSW, Australia.
University of Miami. Rosenstiel School of Marine and Atmospheric Sciences. Miami, FL, USA.
National and Kapodistrian University of Athens. Medical School. Department of Microbiology. Mycology Laboratory. Athens, Greece.
Instituto Nacional de Salud. Grupo de Microbiología. Bogotá, Colombia.
Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.
National and Kapodistrian University of Athens. Medical School. Department of Microbiology. Mycology Laboratory. Athens, Greece.
St. Vincent’s Hospital. Department of Microbiology and Clinical Infectious Diseases. Sydney, NSW, Australia.
University of Sydney. Sydney Medical School-Westmead. Westmead Millennium Institute. Centre for Infectious Diseases and Microbiology / University of Sydney. Sydney Emerging Infections and Biosecurity Institute. Sydney, NSW, Australia.
University of Sydney. Sydney Medical School-Westmead. Westmead Millennium Institute. Centre for Infectious Diseases and Microbiology / University of Sydney. Sydney Emerging Infections and Biosecurity Institute. Sydney, NSW, Australia / Westmead Hospital. Molecular Mycology Research Laboratory. Sydney, NSW, Australia.
Resumo em Inglês
Cryptococcusneoformans and Cryptococcus gattii are pathogenic yeasts causing meningoencephalitis
in immunocompromised and immunocompetent hosts. The fungus is typically haploid, and sexual reproduction occurs normally between individuals with opposite mating types, a and a. C. neoformans var. grubii
(serotype A) is comprised of molecular types VNI, VNII, and VNB, and C. neoformans var. neoformans
(serotype D) contains the molecular type VNIV. Additionally, diploid or aneuploid AD hybrids (VNIII) have been reported. C. gattii contains the molecular types VGI, VGII, VGIII, and VGIV, which encompass both serotypes B and C. To identify possible hybrid strains, URA5-RFLP analysis was performed on 350 globally obtained clinical, environmental, and veterinary isolates. Four clinical isolates from cerebrospinal fluid showed combination patterns of C. neoformans var. grubii and C. gattii: Brazil (n = 2), Colombia (n = 1), and India (n = 1). These strains were monokaryotic and diploid or aneuploid. M13 PCR fingerprinting showed that they contained fragments of both proposed parental groups. Luminex IGS genotyping identified these isolates as hybrids with two different molecular type combinations: three VNI/VGII and one VNI/VGI. Blue color development on CGB agar was delayed in three isolates and absent in one. C. gattii-specific PCR confirmed the presence of C. gattii in the hybrids. CAP59 allele-specific PCR revealed that all the hybrids contained both serotype A and B alleles. Determination of mating-type allelic patterns by PCR revealed that the isolates were aA aB. This is the first study discovering novel natural hybrids between C. neoformans molecular type VNI and C. gattii molecular type VGII.
Palavras-chave em inglês
Cryptococcus neoformansCryptococcus gattii
AB hybrids
Serotype
Mating type
Molecular type
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