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ArtículoDerechos de autor
Acceso abierto
Fecha del embargo
2020-09-26
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- INI - Artigos de Periódicos [3460]
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FIRST MOLECULAR TYPING OF CRYPTOCOCCEMIA-CAUSING CRYPTOCOCCUS IN CENTRAL-WEST BRAZIL
Cryptococcus neoformans
Cryptococcus gattii
Molecular typing
HIV-negative
Autor
Afiliación
Federal University of Mato Grosso do Sul. Biology and Health Sciences Center. Microbiological Research Laboratory. Campo Grande, MS, Brazil.
Federal University of Mato Grosso do Sul. Medical School. Campo Grande, MS, Brazil.
Federal Institute of Mato Grosso do Sul. Nova Andradina. MS, Brazil.
Federal University of Mato Grosso do Sul. Biology and Health Sciences Center. Microbiological Research Laboratory. Campo Grande, MS, Brazil.
Federal University of Mato Grosso do Sul. Biology and Health Sciences Center. Microbiological Research Laboratory. Campo Grande, MS, Brazil.
University Hospital Group. Hospital Universitário Maria Aparecida Pedrossian. Mycology Laboratory. Campo Grande, MS, Brazil.
Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.
Federal University of Mato Grosso do Sul. Biology and Health Sciences Center. Microbiological Research Laboratory. Campo Grande, MS, Brazil.
Federal University of Mato Grosso do Sul. Medical School. Campo Grande, MS, Brazil.
Federal Institute of Mato Grosso do Sul. Nova Andradina. MS, Brazil.
Federal University of Mato Grosso do Sul. Biology and Health Sciences Center. Microbiological Research Laboratory. Campo Grande, MS, Brazil.
Federal University of Mato Grosso do Sul. Biology and Health Sciences Center. Microbiological Research Laboratory. Campo Grande, MS, Brazil.
University Hospital Group. Hospital Universitário Maria Aparecida Pedrossian. Mycology Laboratory. Campo Grande, MS, Brazil.
Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.
Federal University of Mato Grosso do Sul. Biology and Health Sciences Center. Microbiological Research Laboratory. Campo Grande, MS, Brazil.
Resumen en ingles
Molecular epidemiology studies on cryptococcemia are limited. This study aimed to describe the clinical features of patients with bloodstream infections by Cryptococcus sp. in a public tertiary hospital in Mato Grosso do Sul, as well as identify the fungus’ molecular type and determine its antifungal susceptibility. Molecular typing was performed using URA5 restriction fragment length polymorphism PCR, and antifungal susceptibility was determined by microdilution method standardized by the Clinical and Laboratory Standards Institute. Over 14 years, 48 patients were diagnosed with cryptococcemia. The majority (72.9 %) was male with a median age of
40 years; 81.3 % of the patients had HIV/AIDS and 72.9 % died. Cryptococcus neoformans was the most commonly isolated species (97.9 %). Molecular analysis identified the genotypes C. neoformans VNI (93.7 %), C. neoformans VNII (4.2 %), and Cryptococcus gattii VGII (2.1 %). In vitro, these fungi were not resistant to fluconazole, itraconazole, voriconazole, and amphotericin B. This is the first description of the molecular types of cryptococcemia agents in central-west Brazil. Its high lethality, especially in HIV-negative patients, suggests that early diagnosis and prompt antifungal therapy are crucial for a good clinical outcome.
Palabras clave en ingles
CryptococcemiaCryptococcus neoformans
Cryptococcus gattii
Molecular typing
HIV-negative
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