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ANTIOXIDANT, ANTIBACTERIAL, LEISHMANICIDAL AND TRYPANOCIDAL ACTIVITIES OF EXTRACT AND FRACTIONS OF MANILKARA RUFULA STEM BARK
Manilkara rufula
Atividades farmacológicas
Composição química
Manilkara rufula
Pharmacological activities
Chemical composition
Autor
Afiliación
State University of the Southwest of Bahia. Department of Biological Sciences. Jequié, BA, Brazil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
State University of the Southwest of Bahia. Department of Sciences and Technologies. Jequié, BA, Brazil.
State University of the Southwest of Bahia. Department of Sciences and Technologies. Jequié, BA, Brazil.
University of São Paulo. Faculty of Animal Science and Food Engineering. Pirassununga, SP, Brazil.
State University of the Southwest of Bahia. Department of Natural Sciences. Vitória da Conquista, BA, Brazil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
State University of the Southwest of Bahia. Department of Sciences and Technologies. Jequié, BA, Brazil.
State University of the Southwest of Bahia. Department of Sciences and Technologies. Jequié, BA, Brazil.
University of São Paulo. Faculty of Animal Science and Food Engineering. Pirassununga, SP, Brazil.
State University of the Southwest of Bahia. Department of Natural Sciences. Vitória da Conquista, BA, Brazil.
Resumen en ingles
Manilkara rufula belongs to family Sapotaceae and has received little attention regarding its pharmacological properties and chemical composition. Therefore, our objective was to determine the farmacological activities and preliminary chemical profile of the stem bark from M. rufula. The plant stem bark of M. rufula was collected and ethanolic extract and fractions were prepared. The antioxidant activity was determined by •DPPH and ABTS•+ scavenger methods and inhibition of β-carotene oxidation. The minimum inhibitory and bactericidal concentrations were estimated through the broth microdilution method against gram positive and negative bacteria. Cytotoxicity, antioxidant and cytoprotection potential were investigated on
culture macrophages. Antiproliferative activity was evaluated by Alamar Blue method. The in vitro arginase activity from Leishmania amazonensis was determined in presence of the samples. In addition, their effects on survival of L. amazonensis and Trypanosoma cruzi amastigotes in mice macrophages were assessed. The preliminary phytochemical profile was evaluated by qualitative methods of classical phytochemistry, infrared
spectroscopy, LC-MS/MS and CG-MS. The samples presented antioxidant, bactericidal, leishmanicidal and trypanocidal properties without toxic characteristics on normal cells. Triterpenes were observed in the hexane
fraction, while glycosides, aromatic rings, fatty esters, proanthocyanidin dimers and trimers, catechin and various terpenes were observed in the other fractions.
Palabras clave en portugues
SapotaceaeManilkara rufula
Atividades farmacológicas
Composição química
Palabras clave en ingles
SapotaceaeManilkara rufula
Pharmacological activities
Chemical composition
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