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2100-01-01
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A NOVEL POLYMER‐BASED GENOSENSOR FOR THE DETECTION AND QUANTIFICATION OF STREPTOCOCCUS PNEUMONIAE IN GENOMIC DNA SAMPLE
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Universidade Federal de Uberlândia. Instituto de Química. Uberlândia, MG, Brasil.
Universidade Federal de Uberlândia. Instituto de Bioquímica. Uberlândia, MG, Brasil.
Universidade Federal de Uberlândia. Instituto de Química. Uberlândia, MG, Brasil.
Fundação Oswaldo Cruz. Instituto Rene Rachou. Centro de Excelência em Bioinformática. Belo Horizonte, MG, Brasil.
Instituto Tecnológico Vale. Belém, PA, Brasil.
Universidade Federal de Uberlândia. Instituto de Química. Uberlândia, MG, Brasil.
Universidade Federal de Uberlândia. Instituto de Bioquímica. Uberlândia, MG, Brasil.
Universidade Federal de Uberlândia. Instituto de Bioquímica. Uberlândia, MG, Brasil.
Universidade Federal de Uberlândia. Instituto de Química. Uberlândia, MG, Brasil.
Fundação Oswaldo Cruz. Instituto Rene Rachou. Centro de Excelência em Bioinformática. Belo Horizonte, MG, Brasil.
Instituto Tecnológico Vale. Belém, PA, Brasil.
Universidade Federal de Uberlândia. Instituto de Química. Uberlândia, MG, Brasil.
Universidade Federal de Uberlândia. Instituto de Bioquímica. Uberlândia, MG, Brasil.
Abstract
The Streptococcus pneumoniae detection plays an important role in the diagnosis and monitoring of pneumococcal diseases. A genosensor based on graphite electrodes modified with polymer was developed for such detection. First, the poly(4-aminophenol) film was electrochemically deposited on a graphite electrode. Afterward, an S. pneumoniae -specific oligonucleotide (Strep1), isolated from conserved regions of the bacterial genome, was immobilized onto the modified electrode surface and used for the test with complementary target oligonucleotide (Strep2) or genomic DNA. The genosensor was evaluated using electrochemical techniques and atomic force microscopy. Poly(4-aminophenol) film caused an increase in probe immobilization, monitoring the guanine oxidation peak. The detection limits obtained using differential pulse voltammetry and electrochemical spectroscopy impedance were 54 and 28 ng mL21, respectively. The novel genosensor was efficient for the immobilization and detection of S. pneumoniae genomic DNA.
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