Panstrongylus rufotuberculatus is widely distributed in Central and South America. In Peru, it has been found in Tumbes, Piura, and Cuzco. The authors report the presence of P. rufotuberculatus in Chirinos-La Pareja, Suyo district, Ayabaca Province, Piura Region, Peru. Twenty-eight specimens were collected in two of 15 dwellings: three 2nd instar, four 3rd instar, two 4th instar, and eight 5th instar nymphs, three male and eight female, in an intra-domiciliary colony of guinea pigs (Cavia porcellus, raised indoors as a source of diet protein) and beds and bedroom walls. None of the fecal samples were naturally infected with Trypanosoma cruzi. This is the first report of synanthropic presence of P. rufotuberculatus in the region. The finding emphasizes the need for careful entomological and epidemiological surveillance of this and other triatomine species in the Region.