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ArtigoDireito Autoral
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2050-01-01
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L-ASPARAGINASE ISOLATED FROM STREPTOMYCES ANSOCHROMOGENES PROMOTES TH1 PROFILE AND ACTIVATES CD8+ T CELLS IN HUMAN PBMC: AN IN VITRO INVESTIGATION
Immunomodulation
L-asparaginase
Lymphocytes
Streptomyces ansochromogenes
Asparaginase / farmacologia
Asparaginase / toxicidade
Linfócitos T CD8-Positivos / efeitos de drogas
Linfócitos T CD8-Positivos / imunologia
Humanos
Leucócitos Mononucleares / efeitos de drogas
Leucócitos Mononucleares / imunologia
Ativação de Linfócitos / efeitos de drogas
Streptomyces / enzimologia
Th1 Células / efeitos de drogas
Autor(es)
Afiliação
Federal University of Pernambuco. Department of Antibiotics. Recife, PE, Brazil.
Federal University of Pernambuco. Department of Antibiotics. Laboratory of Immunological and Antitumor Analyzes. Recife, PE, Brazil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Laboratório de Imunoparasitologia. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Laboratório de Imunoparasitologia. Recife, PE, Brasil.
Federal University of Pernambuco. Department of Biochemistry. Laboratory of Glycoproteins. Recife, PE, Brasil.
Federal University of Pernambuco. Department of Antibiotics. Recife, PE, Brazil.
Federal University of Pernambuco. Department of Biochemistry. Laboratory of Glycoproteins. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Laboratório de Imunoparasitologia.
Federal University of Pernambuco. Department of Antibiotics. Recife, PE, Brazil.
Federal University of Pernambuco. Department of Antibiotics. Recife, PE, Brazil.
Federal University of Pernambuco. Department of Antibiotics. Laboratory of Immunological and Antitumor Analyzes. Recife, PE, Brazil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Laboratório de Imunoparasitologia. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Laboratório de Imunoparasitologia. Recife, PE, Brasil.
Federal University of Pernambuco. Department of Biochemistry. Laboratory of Glycoproteins. Recife, PE, Brasil.
Federal University of Pernambuco. Department of Antibiotics. Recife, PE, Brazil.
Federal University of Pernambuco. Department of Biochemistry. Laboratory of Glycoproteins. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Laboratório de Imunoparasitologia.
Federal University of Pernambuco. Department of Antibiotics. Recife, PE, Brazil.
Federal University of Pernambuco. Department of Antibiotics. Recife, PE, Brazil.
Resumo em Inglês
Aims:A new L-asparaginase produced byStreptomyces ansochromogenesUFPEDA 3420 actinobacteria was used in this study against humanlymphocyte cultures to evaluate the immunological profile induced by thisenzyme.Methods and Results:Cultures of lymphocytes were stimulated withS.ansochromogenesL-asparaginase, and cytotoxicity, cell viability, cell stimulationand cytokine production were analysed. This newS. ansochromogenesL-asparaginase induced activation and proliferation of the TCD8+lymphocytesubset and produced higher TNF-a, IFN-c, IL-2 and IL-10 levels in a 24-hassay.Conclusion:Streptomyces ansochromogenesL-asparaginase is a promisingmolecule to be used inin vivomodels and to deepen preclinical tests againstacute lymphoblast leukaemia.Significance and Impact of Study:L-asparaginase is an indispensablecomponent of the chemotherapeutic treatment of acute lymphoblast leukaemia(ALL) and acute myeloid leukaemia (AML). Currently, drugs such asAsparaginaseâ, Kidrolaseâ, and Elsparâand Erwinaseâare efficient againstleukemic disease, but promote immunosuppression and other side effects inhuman organisms. Our purifiedS. ansochromogenesL-asparaginase showedpromissory results inducing,in vitro, higher immunostimulation in humanPBMC, especially in T CD8+lymphocyte subsets.
Palavras-chave em inglês
Acute lymphoblast leukaemiaImmunomodulation
L-asparaginase
Lymphocytes
Streptomyces ansochromogenes
DeCS
Asparaginase / isolamento e purificaçãoAsparaginase / farmacologia
Asparaginase / toxicidade
Linfócitos T CD8-Positivos / efeitos de drogas
Linfócitos T CD8-Positivos / imunologia
Humanos
Leucócitos Mononucleares / efeitos de drogas
Leucócitos Mononucleares / imunologia
Ativação de Linfócitos / efeitos de drogas
Streptomyces / enzimologia
Th1 Células / efeitos de drogas
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