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BIOLOGICAL FUNCTION AND MOLECULAR MAPPING OF M ANTIGEN IN YEAST PHASE OF HISTOPLASMA CAPSULATUM
Antígeno M
Função Biológica
Mapeamento Molecular
Fase de Levedura
M antigen
Biological Function
Molecular Mapping
Yeast Phase
Autor
Afiliación
Albert Einstein College of Medicine of Yeshiva University. Department of Medicine and Microbiology and Immunology. Division of Infectious Diseases. Bronx, NY, USA / Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Setor de Imunodiagnóstico. Rio de Janeiro, RJ, Brasil.
St John’s Institute of Dermatology. Guy’s Hospital. King’s College, London, United Kingdom.
Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Setor de Imunodiagnóstico. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica de Proteínas e Peptídeos. Rio de Janeiro, RJ, Brasil.
Albert Einstein College of Medicine of Yeshiva University. Department of Medicine and Microbiology and Immunology. Division of Infectious Diseases. Bronx, NY, USA.
Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Setor de Imunodiagnóstico. Rio de Janeiro, RJ, Brasil.
St John’s Institute of Dermatology. Guy’s Hospital. King’s College, London, United Kingdom.
Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Setor de Imunodiagnóstico. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica de Proteínas e Peptídeos. Rio de Janeiro, RJ, Brasil.
Albert Einstein College of Medicine of Yeshiva University. Department of Medicine and Microbiology and Immunology. Division of Infectious Diseases. Bronx, NY, USA.
Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Setor de Imunodiagnóstico. Rio de Janeiro, RJ, Brasil.
Resumen en ingles
Histoplasmosis, due to the intracellular fungus Histoplasma capsulatum, can be diagnosed by demonstrating the presence of antibodies specific to the immunodominant M antigen. However, the role of this protein in the pathogenesis of histoplasmosis has not been elucidated. We sought to structurally and immunologically characterize the protein, determine yeast cell surface expression, and confirm catalase activity. A 3D-rendering of the M antigen by homology modeling revealed that the structures and domains closely resemble characterized fungal catalases. We generated monoclonal antibodies (mAbs) to the protein and determined that the M antigen is present on the yeast cell surface and in cell wall/cell membrane preparations. Similarly, we found that the majority of catalase activity was in extracts containing fungal surface antigens and that the M antigen is not significantly secreted by live yeast cells. The mAbs also identified unique epitopes on the M antigen. The localization of the M antigen to the cell surface of H. capsulatum yeast and the characterization of the protein’s major epitopes have important implications since it demonstrates that although the protein may participate in protecting the fungus against oxidative stress it is also accessible to host immune cells and antibody.
Palabras clave en portugues
Histoplasma capsulatumAntígeno M
Função Biológica
Mapeamento Molecular
Fase de Levedura
Palabras clave en ingles
Histoplasma capsulatumM antigen
Biological Function
Molecular Mapping
Yeast Phase
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