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TOLL LIKE RECEPTORS HAVE MRNA DIFFERENTIATED EXPRESSION IN DENDRITIC CELLS IN CRISIS-STATE SICKLE CELL ANEMIA PATIENTS, SUGGESTING A PIVOTAL ROLE OF THESE MOLECULES AND CELL TYPE IN THE MAINTENANCE OF INFLAMMATORY RESPONSE
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Abstract
The vascular alterations play an important role in the SCA chronic inflammatory state
with the participation of different cell types, including macrophages and dendritic cells (DCs).
Aim Here, we evaluated the mRNA expression of TLRs in DCs cells pre and post LPS stimulus and
also measured IL-1β, TNF-α and IL-10 cytokines in plasma and supernatant of DCs pre and post
LPS challenge, in order to indentify a possible role of this cell and receptor in the inflammatory
state described in sickle cell anemia.
Methods In vitro generation of immature DCs (imDC) monocyte-derived was obtained of
monocytes from peripheral blood mononuclear cells (PBMC) of seven hospitalized HbSS patients. Relative mRNA abundance of each transcript was investigated by quantitative real time PCR and cytokines levels were measured by ELISA.
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