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https://www.arca.fiocruz.br/handle/icict/32292
RESIALYLATION OF SIALIDASE-TREATED SHEEP AND HUMAN ERYTHROCYTES BY TRYPANOSOMA CRUZI TRANS-SIALIDASE: RESTORATION OF COMPLEMENT RESISTANCE OF DESIALYLATED SHEEP ERYTHROCYTES
Eritrócitos
Complemento
Ácido sialidase
Acido sialico
Trans-sialidase
Affilliation
New York University Medical Center. Department of Pathology. New York, NY, USA.
New York University Medical Center. Department of Pathology. New York, NY, USA.
New York University Medical Center. Department of Pathology. New York, NY, USA.
New York University Medical Center. Department of Pathology. New York, NY, USA.
New York University Medical Center. Department of Pathology. New York, NY, USA.
New York University Medical Center. Department of Pathology. New York, NY, USA.
New York University Medical Center. Department of Pathology. New York, NY, USA.
Abstract
Trypanosoma cruzi trans-sialidase (TS) is a recently
described enzyme whkh transfers a(2-3)-linked sialic acid
from host-derived sialylated glycoconjugates to parasite
surface molecules [Schenkman et al. (1991) Cell, 65, 1117].
We report here on the ability of TS to transfer sialic acid
from donor sialyl-a(2-3)lactose to sialidase-treated sheep
and human erythrocytes. Up to ~ 50% resialylation of both
desialylated red cells could be attained. Resialylation of
desialylated sheep erythrocytes restores their resistance to
lysis by human complement. This ascribes a possible
biological role for T.cruzi TS and demonstrates directly that
sialic acid is solely responsible for preventing alternative
pathway activation of human complement by sheep
erythrocytes.
Keywords in Portuguese
Trypanosoma cruziEritrócitos
Complemento
Ácido sialidase
Acido sialico
Trans-sialidase
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