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2022-01-01
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NON-SPECIFIC ANTIVIRAL RESPONSE DETECTED IN RNA-TREATED CULTURED CELLS OF THE SANDFLY, LUTZOMYIA LONGIPALPIS
Resposta antiviral
Vírus do Nilo Ocidental
Partícula semelhante a vírus
Imunidade inata
Silenciamento de genes
LL5 cells
Antiviral response
West Nile virus
Virus-like particle
Innate immunity
siRNA
Gene silencing
Affilliation
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Tripanosomatídeos e Flebetomineos. Rio de Janeiro, RJ, Brasil / University of Texas Medical Branch. Department of Pathology and Sealy Center for Vaccine Development. Galveston, TX, USA.
University of Texas Medical Branch. Department of Pathology and Sealy Center for Vaccine Development. Galveston, TX, USA.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Tripanosomatídeos e Flebetomineos. Rio de Janeiro, RJ, Brasil.
University of Texas Medical Branch. Department of Pathology and Sealy Center for Vaccine Development. Galveston, TX, USA.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Tripanosomatídeos e Flebetomineos. Rio de Janeiro, RJ, Brasil.
Abstract
Lutzomyia longipalpis is the principal vector of visceral leishmaniasis in the Americas, and can also transmit some viruses. To help develop a gene-silencing system for this sandfly, we transfected cultured embryonic cells with various double-stranded RNAs using West Nile virus (WNV) virus-like particles (VLPs) expressing luciferase as the target RNA to demonstrate effective gene knock-down. When luciferase dsRNA was introduced into these cells, they produced the expected reduction in VLP-encoded luciferase, suggesting specific silencing of the luciferase gene. Surprisingly, we found that unrelated dsRNAs, which included those specific for several L. longipalpis gene sequences and Escherichia coli beta-galactosidase, diminished replication of the VLP-encoded genome. These results are the first indication for a nucleic acid-induced, non-specific antiviral response in this important insect vector.
Keywords in Portuguese
Lutzomyia LongipalpisResposta antiviral
Vírus do Nilo Ocidental
Partícula semelhante a vírus
Imunidade inata
Silenciamento de genes
Keywords
Lutzomyia LongipalpisLL5 cells
Antiviral response
West Nile virus
Virus-like particle
Innate immunity
siRNA
Gene silencing
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