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https://www.arca.fiocruz.br/handle/icict/31797
GENE EXPRESSION ANALYSIS OF HUMAN ADIPOSE TISSUE-DERIVED STEM CELLS DURING THE INITIAL STEPS OF IN VITRO OSTEOGENESIS
Mesenchymal stem cells
Osteogenesis
Gene Expression Regulation
Osteogénesis
Regulación de la Expresión Génica
Autor(es)
Afiliação
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Instituto Pasteur. Unidade de Bioinformática. Montevideo, Uruguay.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Cirurgia Plástica Dr. Paulo Bettes. Curitiba, PR, Brasil.
Instituto Pasteur. Unidade de Bioinformática. Montevideo, Uruguay.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Instituto Pasteur. Unidade de Bioinformática. Montevideo, Uruguay.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Cirurgia Plástica Dr. Paulo Bettes. Curitiba, PR, Brasil.
Instituto Pasteur. Unidade de Bioinformática. Montevideo, Uruguay.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
Resumo em Inglês
Mesenchymal stem cells (MSCs) have been widely studied with regard to their potential use in cell therapy protocols and regenerative medicine. However, a better comprehension about the factors and molecular mechanisms driving cell differentiation is now mandatory to improve our chance to manipulate MSC behavior and to benefit future applications. In this work, we aimed to study gene regulatory networks at an early step of osteogenic differentiation. Therefore, we analyzed both the total mRNA and the mRNA fraction associated with polysomes on human adipose tissue-derived stem cells (hASCs) at 24 h of osteogenesis induction. The RNA-seq results evidenced that hASC fate is not compromised with osteogenesis at this time and that 21 days of continuous cell culture stimuli are necessary for full osteogenic differentiation of hASCs. Furthermore, early stages of osteogenesis induction involved gene regulation that was linked to the management of cell behavior in culture, such as the control of cell adhesion and proliferation. In conclusion, although discrete initial gene regulation related to osteogenesis occur, the first 24 h of induction is not sufficient to trigger and drive in vitro osteogenic differentiation of hASCs.
Palavras-chave
Células-tronco mesenquimaisPalavras-chave em inglês
Mesenchymal Stromal CellsMesenchymal stem cells
Osteogenesis
Gene Expression Regulation
Palavras-chave em espanhol
Células del Estroma MesenquimatosoOsteogénesis
Regulación de la Expresión Génica
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