Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/30232

Type
Article
Copyright
Restricted access
Embargo date
2030-01-01
Sustainable Development Goals
03 Saúde e Bem-Estar
Collections
Share

Statistics
Metadata
Show full item record

ANALYSIS OF LEISHMANIA CHAGASI BY 2-D DIFFERENCE GEL ELETROPHORESIS (2-D DIGE) AND IMMUNOPROTEOMIC: IDENTIFICATION OF NOVEL CANDIDATE ANTIGENS FOR DIAGNOSTIC TESTS AND VACCINE
Costa, Míriam M. et al. | Date Issued: 2011
Author
Costa, Míriam M.
Andrade, Hélida M.
Bartholomeu, Daniella C.
Freitas, Leandro M.
Pires, Simone F.
Chapeaurouge, Alexander D.
Perales, Jonas
Ferreira, André T.
Giusta, Mário S.
Melo, Maria N.
Gazzinelli, Ricardo T.
Affilliation
Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MiG, Brasil.
Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil.
Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil.
Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil.
Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ. Brasil.
Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MiG, Brasil.
Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil.
Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MiG, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.
University of Massachusetts Medical School. Division of Infectious Diseases and Immunology. Massachussets, USA.
Abstract
Identification of novel antigens is essential for developing new diagnostic tests and vaccines. We used DIGE to compare protein expression in amastigote and promastigote forms of Leishmania chagasi. Nine hundred amastigote and promastigote spots were visualized. Five amastigote-specific, 25 promastigote-specific, and 10 proteins shared by the two parasite stages were identified. Furthermore, 41 proteins were identified in the Western blot employing 2-DE and sera from infected dogs. From these proteins, 3 and 38 were reactive with IgM and total IgG, respectively. The proteins recognized by total IgG presented different patterns in terms of their recognition by IgG1 and/or IgG2 isotypes. All the proteins selected by Western blot were mapped for B-cell epitopes. One hundred and eighty peptides were submitted to SPOT synthesis and immunoassay. A total of 25 peptides were shown of interest for serodiagnosis to visceral leishmaniasis. In addition, all proteins identified in this study were mapped for T cell epitopes by using the NetCTL software, and candidates for vaccine development were selected. Therefore, a large-scale screening of L. chagasi proteome was performed to identify new B and T cell epitopes with potential use for developing diagnostic tests and vaccines.
Keywords in Portuguese
Leishmaniose
Proteoma
Ántígenos
Diagnóstico
Vacina
 
Keywords
Leishmaniasis
proteome
antigens
diagnosis
vaccine
 
Publisher
American Chemical Society
Citation
COSTA, Míriam M. et al. Analysis of Leishmania chagasi by 2-D Difference Gel Eletrophoresis (2-D DIGE) and Immunoproteomic: Identification of Novel Candidate Antigens for Diagnostic Tests and Vaccine. J. Proteome Res., v.10, p.2172–2184, Feb. 2011.
DOI
10.1021/pr101286y
ISSN
1535-3893