Use este identificador para citar ou linkar para este item:
https://www.arca.fiocruz.br/handle/icict/28857
Tipo de documento
ArtigoDireito Autoral
Acesso restrito
Data de embargo
2024-01-01
Coleções
Metadata
Mostrar registro completo
IMPLICATIONS OF THE USE OF SEROLOGICAL AND MOLECULAR METHODS TO DETECT INFECTION BY LEISHMANIA SPP. IN URBAN PET DOGS.
Leishmania amazonensis
Leishmania infantum
cachorros
ensaios sorologicos
Leishmania amazonensis
Leishmania infantum
Dogs
Serological assays
Autor(es)
Afiliação
Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Estudos em Leishmanioses. Belo Horizonte, MG, Brazil
Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Estudos em Leishmanioses. Belo Horizonte, MG, Brazil
Fundação Ezequiel Dias. Divisão de Epidemiologia e Controle de Doenças. Serviço de Doenças Parasitárias. Belo Horizonte, MG, Brazil
Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Estudos em Leishmanioses. Belo Horizonte, MG, Brazil
Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Estudos em Leishmanioses. Belo Horizonte, MG, Brazil
Fundação Ezequiel Dias. Divisão de Epidemiologia e Controle de Doenças. Serviço de Doenças Parasitárias. Belo Horizonte, MG, Brazil
Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Estudos em Leishmanioses. Belo Horizonte, MG, Brazil
Resumo em Inglês
The aim of this study was to evaluate the relationship between naturally occurring Leishmania spp. infections in dogs (Canis familiaris) and the practical implications of the use of serological and molecular methods to confirm diagnoses. The study population consisted of 96 domestic dogs in southeastern Brazil. Serum samples were tested for the presence of anti-Leishmania immunoglobulin G (IgG) antibodies using four commercial canine visceral leishmaniasis kits. Dogs confirmed positive by immunofluorescence antibody test (IFAT) were culled and samples from mesenteric lymph nodes, spleen border, bone marrow and ear skin were taken and submitted to DNA extraction. PCR reactions were performed using primers that amplify a 300–350 bp fragment of the Leishmania ribosomal internal transcribed spacer 1 (ITS1) region. The ITS1 amplified products were analyzed by PCR-RFLP using Hae III restriction endonuclease. To confirm the Leishmania species detected by PCR, each purified sample was sequenced in duplicate. Of the 96 serum samples submitted to serological assays, 8 (8.3%) tested positive for Leishmania by IFAT, 4 (4.1%) by ELISA, 2 (2.1%) by rK39 RDT and 7 (7.3%) by DPP. Four of these infected dogs (50%) were found to be infected only by Leishmania braziliensis or Leishmania amazonensis, and their serum samples tested positive by IFAT and DPP. These findings demonstrate for the first time that cross-reactivity of L. braziliensis and L. amazonensis infection in dogs can be found using the DPP serum test. This is the first record of Leishmania (Leishmania) amazonensis confirmed by a specific molecular marker in dogs (Canis familiaris) from Belo Horizonte, Brazil.
Palavras-chave
Leishmania braziliensisLeishmania amazonensis
Leishmania infantum
cachorros
ensaios sorologicos
Palavras-chave em inglês
Leishmania braziliensisLeishmania amazonensis
Leishmania infantum
Dogs
Serological assays
Compartilhar