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https://www.arca.fiocruz.br/handle/icict/28498
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2050-01-01
Sustainable Development Goals
03 Saúde e Bem-EstarCollections
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IMMUNIZATION OF NEONATAL MICE WITH LAMP/P55 HIV GAG DNA ELICITS ROBUST IMMUNE RESPONSES THAT LAST TO ADULTHOOD
Vacinas contra a SIDA / imunologia
Infecções por HIV / imunologia
Infecções por HIV / prevenção & controle
HIV -1 / genética
HIV -1 / imunologia
Imunização
Imunização Secundária
Proteína de Membrana Associada aos Lisossomos 1 / genética
Proteína 1 de Membrana Associada a Lisossomos / imunologia
Precursores de Proteína / genética
Precursores de Proteína / imunologia
Vacinas, DNA / administração & dosagem
Ratos , endogâmicos BALB C
Author
Affilliation
University of São Paulo. School of Medicine. Laboratory of Dermatology and Immunodeficiency, LIM-56. São Paulo, SP, Brazil.
University of Maryland. Center for Vaccine Development. Baltimore, USA.
University of São Paulo. School of Medicine. Laboratory of Dermatology and Immunodeficiency, LIM-56. São Paulo, SP, Brazil.
University of São Paulo. School of Medicine. Laboratory of Dermatology and Immunodeficiency, LIM-56. São Paulo, SP, Brazil.
University of São Paulo. School of Medicine. Laboratory of Dermatology and Immunodeficiency, LIM-56. São Paulo, SP, Brazil.
University of São Paulo. School of Medicine. Laboratory of Dermatology and Immunodeficiency, LIM-56. São Paulo, SP, Brazil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Laboratório de Virologia e Terapia Experimental. Recife, PE, Brasil.
The Johns Hopkins School of Medicine. Department of Pharmacology and Molecular Sciences. Baltimore, USA.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Laboratório de Virologia e Terapia Experimental. Recife, PE, Brasil / Center for Vaccine Research. Department of Infectious Diseases and Microbiology. Pittsburg, USA.
University of São Paulo. School of Medicine. Laboratory of Dermatology and Immunodeficiency, LIM-56. São Paulo, SP, Brazil.
University of São Paulo. School of Medicine. Laboratory of Dermatology and Immunodeficiency, LIM-56. São Paulo, SP, Brazil.
University of Maryland. Center for Vaccine Development. Baltimore, USA.
University of São Paulo. School of Medicine. Laboratory of Dermatology and Immunodeficiency, LIM-56. São Paulo, SP, Brazil.
University of São Paulo. School of Medicine. Laboratory of Dermatology and Immunodeficiency, LIM-56. São Paulo, SP, Brazil.
University of São Paulo. School of Medicine. Laboratory of Dermatology and Immunodeficiency, LIM-56. São Paulo, SP, Brazil.
University of São Paulo. School of Medicine. Laboratory of Dermatology and Immunodeficiency, LIM-56. São Paulo, SP, Brazil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Laboratório de Virologia e Terapia Experimental. Recife, PE, Brasil.
The Johns Hopkins School of Medicine. Department of Pharmacology and Molecular Sciences. Baltimore, USA.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Laboratório de Virologia e Terapia Experimental. Recife, PE, Brasil / Center for Vaccine Research. Department of Infectious Diseases and Microbiology. Pittsburg, USA.
University of São Paulo. School of Medicine. Laboratory of Dermatology and Immunodeficiency, LIM-56. São Paulo, SP, Brazil.
University of São Paulo. School of Medicine. Laboratory of Dermatology and Immunodeficiency, LIM-56. São Paulo, SP, Brazil.
Abstract
Successful T cell priming in early postnatal life that can generate effective long-lasting responses until adulthood is critical in HIV vaccination strategies because it prevents early sexual initiation and breastfeeding transmission of HIV. A chimeric DNA vaccine encoding p55 HIV gag associated with lysosome-associated membrane protein 1 (LAMP-1; which drives the antigen to the MIIC compartment), has been used to enhance cellular and humoral antigen-specific responses in adult mice and macaques. Herein, we investigated LAMP-1/gag vaccine immunogenicity in the neonatal period in mice and its ability to generate long-lasting effects. Neonatal vaccination with chimeric LAMP/gag generated stronger Gag-specific immune responses, as measured by the breadth of the Gag peptide-specific IFN-gamma, proliferative responsiveness, cytokine production and antibody production, all of which revealed activation of CD4+ T cells as well as the generation of a more robust CTL response compared to gag vaccine alone. To induce long-lived T and B cell memory responses, it was necessary to immunize neonates with the chimeric LAMP/gag DNA vaccine. The LAMP/gag DNA vaccine strategy could be particularly useful for generating an anti-HIV immune response in the early postnatal period capable of inducing long-term immunological memory.
DeCS
Vacinas contra a AIDS / genéticaVacinas contra a SIDA / imunologia
Infecções por HIV / imunologia
Infecções por HIV / prevenção & controle
HIV -1 / genética
HIV -1 / imunologia
Imunização
Imunização Secundária
Proteína de Membrana Associada aos Lisossomos 1 / genética
Proteína 1 de Membrana Associada a Lisossomos / imunologia
Precursores de Proteína / genética
Precursores de Proteína / imunologia
Vacinas, DNA / administração & dosagem
Ratos , endogâmicos BALB C
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