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https://www.arca.fiocruz.br/handle/icict/28192
Tipo de documento
ArtigoDireito Autoral
Acesso restrito
Data de embargo
2030-01-01
Coleções
- IOC - Artigos de Periódicos [12642]
Metadata
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IN VIVO MYOGENIC POTENTIAL OF HUMAN CD133+ MUSCLE-DERIVED STEM CELLS: A QUANTITATIVE STUDY
Potencial miogênico
in vivo
CD133
Estudo quantitativo
Terapia celular
Myogenic stem cells
In Vivo
Quantitative study
Cellular therapy
Autor(es)
Afiliação
UPMC Université Paris 6, UMR S 974 and UMR S 787. Institut de Myologie, Paris, France / CNRS UMR 7215. Institut de Myologie. Paris, France.
UPMC Université Paris 6, UMR S 974 and UMR S 787. Institut de Myologie, Paris, France / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil.
UPMC Université Paris 6, UMR S 974 and UMR S 787. Institut de Myologie, Paris, France / CNRS UMR 7215. Institut de Myologie. Paris, France.
Università degli Studi di Milano. Centro Dino Ferrari. Fondazione IRCCS Ospedale Maggiore Policlinico, Mangiagalli e Regina Elena. Department of Neurological Sciences. Stem Cell Laboratory. Milan, Italy.
Università degli Studi di Milano. Centro Dino Ferrari. Fondazione IRCCS Ospedale Maggiore Policlinico, Mangiagalli e Regina Elena. Department of Neurological Sciences. Stem Cell Laboratory. Milan, Italy.
Institut Pasteur. Unité Cytokines et Développement Lymphoïde, INSERM U 668. Paris, France.
Università degli Studi di Milano. Centro Dino Ferrari. Fondazione IRCCS Ospedale Maggiore Policlinico, Mangiagalli e Regina Elena. Department of Neurological Sciences. Stem Cell Laboratory. Milan, Italy.
UPMC Université Paris 6, UMR S 974 and UMR S 787. Institut de Myologie, Paris, France / CNRS UMR 7215. Institut de Myologie. Paris, France.
UPMC Université Paris 6, UMR S 974 and UMR S 787. Institut de Myologie, Paris, France / CNRS UMR 7215. Institut de Myologie. Paris, France.
UPMC Université Paris 6, UMR S 974 and UMR S 787. Institut de Myologie, Paris, France / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil.
UPMC Université Paris 6, UMR S 974 and UMR S 787. Institut de Myologie, Paris, France / CNRS UMR 7215. Institut de Myologie. Paris, France.
Università degli Studi di Milano. Centro Dino Ferrari. Fondazione IRCCS Ospedale Maggiore Policlinico, Mangiagalli e Regina Elena. Department of Neurological Sciences. Stem Cell Laboratory. Milan, Italy.
Università degli Studi di Milano. Centro Dino Ferrari. Fondazione IRCCS Ospedale Maggiore Policlinico, Mangiagalli e Regina Elena. Department of Neurological Sciences. Stem Cell Laboratory. Milan, Italy.
Institut Pasteur. Unité Cytokines et Développement Lymphoïde, INSERM U 668. Paris, France.
Università degli Studi di Milano. Centro Dino Ferrari. Fondazione IRCCS Ospedale Maggiore Policlinico, Mangiagalli e Regina Elena. Department of Neurological Sciences. Stem Cell Laboratory. Milan, Italy.
UPMC Université Paris 6, UMR S 974 and UMR S 787. Institut de Myologie, Paris, France / CNRS UMR 7215. Institut de Myologie. Paris, France.
UPMC Université Paris 6, UMR S 974 and UMR S 787. Institut de Myologie, Paris, France / CNRS UMR 7215. Institut de Myologie. Paris, France.
Resumo em Inglês
In recent years, numerous reports have identified in
mouse different sources of myogenic cells distinct from
satellite cells that exhibited a variable myogenic potential
in vivo. Myogenic stem cells have also been described in
humans, although their regenerative potential has rarely
been quantified. In this study, we have investigated
the myogenic potential of human muscle–derived cells
based on the expression of the stem cell marker CD133
as compared to bona fide satellite cells already used in
clinical trials. The efficiency of these cells to participate
in muscle regeneration and contribute to the renewal
of the satellite cell pool, when injected intramuscularly,
has been evaluated in the Rag2−/− γC−/− C5−/− mouse
in which muscle degeneration is induced by cryoinjury.
We demonstrate that human muscle–derived CD133+
cells showed a much greater regenerative capacity
when compared to human myoblasts. The number of
fibers expressing human proteins and the number of
human cells in a satellite cell position are all dramatically
increased when compared to those observed after injection
of human myoblasts. In addition, CD133+/CD34+
cells exhibited a better dispersion in the host muscle
when compared to human myoblasts. We propose that
muscle-derived CD133+ cells could be an attractive
candidate
for cellular therapy.
Palavras-chave
Células-tronco miogênicasPotencial miogênico
in vivo
CD133
Estudo quantitativo
Terapia celular
Palavras-chave em inglês
Human CD133+ Muscle-derived Stem CellMyogenic stem cells
In Vivo
Quantitative study
Cellular therapy
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