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2030-01-01
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- IOC - Artigos de Periódicos [12596]
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TRYPANOSOMA CRUZI INFECTION RESULTS IN THE REDUCED EXPRESSION OF CAVEOLIN-3 IN THE HEART
Author
Affilliation
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultraestrutura Celular. Rio de Janeiro, RJ, Brasil / Albert Einstein College of Medicine. Department of Pathology. Bronk, NY, USA / Albert Einstein College of Medicine. Dominik Purpura Department of Neuroscience. Bronx, NY, USA.
Thomas Jefferson University. Department of Stem Cell Biology & Regenerative Medicine and the Kimmel Cancer Center. Philadelphia, PA, USA / University of Genoa and G. Gaslini Pediatric Institute. The Muscular and Neurodegenerative Disease Unit. Genoa, Italy.
Albert Einstein College of Medicine. Department of Medicine. Bronx, NY, USA / Albert Einstein College of Medicine. Dominik Purpura Department of Neuroscience. Bronx, NY, USA.
Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultraestrutura Celular. Rio de Janeiro, RJ, Brasil.
Albert Einstein College of Medicine. Department of Pathology. Bronk, NY, USA / Albert Einstein College of Medicine. Department of Medicine. Bronx, NY, USA.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultraestrutura Celular. Rio de Janeiro, RJ, Brasil.
Thomas Jefferson University. Department of Stem Cell Biology & Regenerative Medicine and the Kimmel Cancer Center. Philadelphia, PA, USA / University of Genoa and G. Gaslini Pediatric Institute. The Muscular and Neurodegenerative Disease Unit. Genoa, Italy.
Albert Einstein College of Medicine. Department of Medicine. Bronx, NY, USA / Albert Einstein College of Medicine. Dominik Purpura Department of Neuroscience. Bronx, NY, USA.
Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultraestrutura Celular. Rio de Janeiro, RJ, Brasil.
Albert Einstein College of Medicine. Department of Pathology. Bronk, NY, USA / Albert Einstein College of Medicine. Department of Medicine. Bronx, NY, USA.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultraestrutura Celular. Rio de Janeiro, RJ, Brasil.
Abstract
Caveolae are motile, membrane-bound compartments that contain a number of molecules that
participate in cell signaling. Caveolins are protein markers of caveolae and function in a variety of
biological processes. Caveolin-3 (Cav-3) is expressed in muscle cells and Cav-3 null mice display
a cardiomyopathic phenotype. Ultrastructural cytochemistry, confocal microscopy and
immunoblotting revealed a reduction in Cav-3 expression and an activation of ERK (extracellularsignal-
regulated kinase) 48 hours after Trypanosoma cruzi infection of cultured cardiac myocytes.
CD-1 mice infected with the Brazil strain of T. cruzi displayed reduced expression of Cav-3 and
activation of ERK 66 days post infection (dpi). By 180 dpi there was a normalization of these
values. These data suggest that the reduction in Cav-3 expression and the activation of ERK
during the early phase of infection may contribute to the pathogenesis of chagasic
cardiomyopathy.
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