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https://www.arca.fiocruz.br/handle/icict/27728
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2025-01-01
Sustainable Development Goals
03 Saúde e Bem-EstarCollections
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DIAGNOSTIC TOOL BASED ON AN HTLV-1-TAX EXPRESSION SYSTEM IN EUKARYOTIC CELLS USING A POXVIRUS VECTOR
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Affilliation
Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil / Fundação HEMOMINAS. Interdisciplinary HTLV Research Group. Belo Horizonte, MG, Brazil.
Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil.
Fundação Osvaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil /Fundação HEMOMINAS. Interdisciplinary HTLV Research Group. Belo Horizonte, MG, Brazil.
Fundação Osvaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil.
Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil / Fundação HEMOMINAS. Interdisciplinary HTLV Research Group. Belo Horizonte, MG, Brazil.
Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil / Fundação HEMOMINAS. Interdisciplinary HTLV Research Group. Belo Horizonte, MG, Brazil
Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil / Fundação HEMOMINAS. Interdisciplinary HTLV Research Group. Belo Horizonte, MG, Brazil.
Fundação HEMOMINAS. Interdisciplinary HTLV Research Group. Belo Horizonte, MG, Brazil.
Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil / Fundação HEMOMINAS. Interdisciplinary HTLV Research Group. Belo Horizonte, MG, Brazil.
Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil.
Fundação Osvaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil /Fundação HEMOMINAS. Interdisciplinary HTLV Research Group. Belo Horizonte, MG, Brazil.
Fundação Osvaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil.
Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil / Fundação HEMOMINAS. Interdisciplinary HTLV Research Group. Belo Horizonte, MG, Brazil.
Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil / Fundação HEMOMINAS. Interdisciplinary HTLV Research Group. Belo Horizonte, MG, Brazil
Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil / Fundação HEMOMINAS. Interdisciplinary HTLV Research Group. Belo Horizonte, MG, Brazil.
Fundação HEMOMINAS. Interdisciplinary HTLV Research Group. Belo Horizonte, MG, Brazil.
Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil / Fundação HEMOMINAS. Interdisciplinary HTLV Research Group. Belo Horizonte, MG, Brazil.
Abstract
Human T-lymphotropic virus 1 (HTLV-1) induces an immune-mediated inflammatory disease affecting the nervous system that eventually is accompanied by ocular, rheumatic and dermatologic manifestations (HTLV-1 associated myelopathy/tropical spastic paraparesis, or HAM/TSP). Proviral load and HTLV-1 protein expression, mainly of Tax, is correlated with disease progression and induction of host–virus equilibrium breakdown that, reportedly, involves the presence of Tax-specific cytotoxic T lymphocytes (CTL), T regulatory cells and anti-Tax antibodies. Based on knowledge of anti-Tax antibodies as markers of disease progression, the objectives of this study were both to design an infection/transfection system using the Vaccinia virus and a tax-encoding plasmid for the expression of Tax protein as well as to use this cell support to evaluate anti-Tax IgG by flow cytometry. The flow cytometry assay was standardized using pooled sera from each test group (negative, asymptomatic and HAM/TSP patients). The HAM/TSP group presented higher IgG anti-Tax reactivity (above 70%) than the asymptomatic group (nearly 40% reactivity). The data indicate that the infection/transfection system is useful for assessing Tax expression. This is a promising assay for use as a diagnostic tool to detect IgG anti-Tax and monitor HTLV-1 infected individuals.
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