Please use this identifier to cite or link to this item:
https://www.arca.fiocruz.br/handle/icict/26749
Type
ArticleCopyright
Restricted access
Embargo date
2024-01-01
Sustainable Development Goals
03 Saúde e Bem-EstarCollections
Metadata
Show full item record
THE MOLECULAR SENSORY MACHINERY OF A CHAGAS DISEASE VECTOR: EXPRESSION CHANGES THROUGH IMAGINAL MOULT AND SEXUALLY DIMORPHIC FEATURES
Author
Affilliation
Fundação Oswaldo Cruz. Instituto René Rachou. Comportamento de Vetores e Interação com Patógenos. Belo Horizonte, MG, Brazil
Department of Entomology. University of Illinois at Urbana. Champaign, Urbana, IL 61801, USA.
Department of Entomology. University of Illinois at Urbana. Champaign, Urbana, IL 61801, USA.
Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Informática de Biossistemas. Belo Horizonte, MG, Brazil Gerais, Brazil
Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Informática de Biossistemas. Belo Horizonte, MG, Brazil Gerais, Brazil
Fundação Oswaldo Cruz. Instituto René Rachou. Comportamento de Vetores e Interação com Patógenos. Belo Horizonte, MG, Brazil
Fundação Oswaldo Cruz. Instituto René Rachou. Comportamento de Vetores e Interação com Patógenos. Belo Horizonte, MG, Brazil
Department of Entomology. University of Illinois at Urbana. Champaign, Urbana, IL 61801, USA.
Department of Entomology. University of Illinois at Urbana. Champaign, Urbana, IL 61801, USA.
Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Informática de Biossistemas. Belo Horizonte, MG, Brazil Gerais, Brazil
Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Informática de Biossistemas. Belo Horizonte, MG, Brazil Gerais, Brazil
Fundação Oswaldo Cruz. Instituto René Rachou. Comportamento de Vetores e Interação com Patógenos. Belo Horizonte, MG, Brazil
Fundação Oswaldo Cruz. Instituto René Rachou. Comportamento de Vetores e Interação com Patógenos. Belo Horizonte, MG, Brazil
Abstract
The triatomine bug Rhodnius prolixus is a main vector of Chagas disease, which affects several million people, mostly in Latin-America. Host searching, pheromone communication, and microclimatic preferences are aspects of its behaviour that depend on multimodal sensory inputs. The molecular bases of these sensory processes are largely unknown. The expression levels of genes transcribed in antennae were compared between 5th instar larvae, and female and male adults by means of RNA-Seq. The antennae of R. prolixus showed increased expression of several chemosensory-related genes in imaginal bugs, while both sexes had similar expression patterns for most target genes. Few cases suggest involvement of target genes in sexually dimorphic functions. Most odorant and ionotropic receptor genes seemed to be expressed in all libraries. OBPs and CSPs showed very high expression levels. Other sensory-related genes such as TRPs, PPKs and mechanoreceptors had consistent levels of expression in all libraries. Our study characterises most of the sensory gene repertoire of these insects, opening an avenue for functional genetics studies. The increase in expression of chemosensory genes suggests na enhanced role in adult bugs. This knowledge allows developing new behaviour interfering strategies, increasing the options for translational research in the vector control field.
Share