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https://www.arca.fiocruz.br/handle/icict/26724
LINALOOL, A PIPER ADUNCUM ESSENTIAL OIL COMPONENT, HAS SELECTIVE ACTIVITY AGAINST TRYPANOSOMA CRUZI TRYPOMASTIGOTE FORMS AT 4°C
Autor(es)
Afiliação
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Celular. Curitiba, PR, Brasil.
Universidade Federal de Lavras. Departamento de Química. Lavras, MG, Brasil.
Universidade Federal de Lavras. Departamento de Química. Lavras, MG, Brasil.
Universidade Federal de Lavras. Departamento de Química. Lavras, MG, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Celular. Curitiba, PR, Brasil.
Universidade Federal de Lavras. Departamento de Química. Lavras, MG, Brasil.
Universidade Federal de Lavras. Departamento de Química. Lavras, MG, Brasil.
Universidade Federal de Lavras. Departamento de Química. Lavras, MG, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Celular. Curitiba, PR, Brasil.
Resumo em Inglês
Recent studies showed that essential oils from different pepper species (Piper spp) have promising leishmanicidal and trypanocidal activities. With this objective was in search for natural compounds against Trypanosoma cruzi, different forms of the parasite were incubated for 24 h at 28ºC or 4ºC with Piper aduncum essential oil (PaEO) or its main constituents linalool and nerolidol. The methods were PaEO chemical composition was obtained by GC-MS. Drug activity assays were based on cell counting, MTT data or infection index values. The effect of PaEO on the T. cruzi cell cycle and mitochondrial membrane potential was evaluated by flow cytometry. Findings PaEO was effective against cell-derived (IC50/24 h: 2.8 μg/mL) and metacyclic (IC50/24 h: 12.1 μg/mL) trypomastigotes, as well as intracellular amastigotes (IC50/24 h: 9 μg/mL). At 4ºC - the temperature of red blood cells (RBCs) storage in blood banks - cell-derived trypomastigotes were more sensitive to PaEO (IC50/24 h = 3.8 μg/mL) than to gentian violet (IC50/24 h = 24.7 mg/mL). Cytotoxicity assays using Vero cells (37ºC) and RBCs (4ºC) showed that PaEO has increased selectivity for cellderived trypomastigotes. Flow cytometry analysis showed that PaEO does not affect the cell cycle of T. cruzi epimastigotes, but decreases their mitochondrial membrane potential. GC-MS data identified nerolidol and linalool as major components of PaEO, and linalool had trypanocidal effect (IC50/24 h: 306 ng/mL) at 4ºC. How main conclusion we have that the trypanocidal effect of PaEO is likely due to the presence of linalool, which may represent an interesting candidate for use in the treatment of potentially contaminated RBCs bags at low temperature.
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