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https://www.arca.fiocruz.br/handle/icict/26398
POLY(ANHYDRIDE) NANOPARTICLES CONTAINING CASHEW NUT PROTEINS CAN INDUCE A STRONG TH1 AND TREG IMMUNE RESPONSE AFTER ORAL ADMINISTRATION
Autor(es)
Afiliação
Federal University of Pernambuco. Immunopathology Keizo-Asami Laboratory. Recife, PE, Brazil
University of Pernambuco. Institute of Biological Sciences. Recife, PE, Brazil
Federal University of Pernambuco. Immunopathology Keizo-Asami Laboratory. Recife, PE, Brazil
University of Navarra. Nanomedicines and Vaccines. Research Group. Pamplona, Spain
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Parasita-Hospedeiro Interação e Epidemiologia. Salvador, BA, Brasil
University of Navarra. Nanomedicines and Vaccines. Research Group. Pamplona, Spain
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Parasita-Hospedeiro Interação e Epidemiologia. Salvador, BA, Brasil
University of Navarra. Nanomedicines and Vaccines. Research Group. Pamplona, Spain
Federal University of Pernambuco. Immunopathology Keizo-Asami Laboratory. Recife, PE, Brazil
University of Pernambuco. Institute of Biological Sciences. Recife, PE, Brazil
Federal University of Pernambuco. Immunopathology Keizo-Asami Laboratory. Recife, PE, Brazil
University of Navarra. Nanomedicines and Vaccines. Research Group. Pamplona, Spain
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Parasita-Hospedeiro Interação e Epidemiologia. Salvador, BA, Brasil
University of Navarra. Nanomedicines and Vaccines. Research Group. Pamplona, Spain
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Parasita-Hospedeiro Interação e Epidemiologia. Salvador, BA, Brasil
University of Navarra. Nanomedicines and Vaccines. Research Group. Pamplona, Spain
Federal University of Pernambuco. Immunopathology Keizo-Asami Laboratory. Recife, PE, Brazil
Resumo em Inglês
Cashew nut allergy is the second most commonly reported tree nut allergy. Traditional allergen immunotherapy presents several clinical drawbacks that can be reduced by using nanoparticles-basedallergen-delivery systems, modulating the immune response towards a protective one. In this context, the goal of this work was to assess the potential of poly(anhydride) nanoparticles (NP) for cashew nut oral immunization. Cashew nut allergens-loaded nanoparticles (CNE-NP) were prepared by solvent displacement method. After nanoparticles characterization, oral immunomodulation ability was evaluated in BALB/c mice. Our results demonstrated that CNE-NP induced a higher Th1/Th2 ratio in comparison with animals immunized with free cashew nut proteins. Indeed, a decrease in splenic Th2 cytokines (IL-4, IL-5, and IL-13), and an enhancement of pro-Th1 (IL-12 and IFN-γ) and regulatory (IL-10) cytokines was observed. Furthermore, mice orally immunized with CNE-NP presented an increased expansion of CD4+ T regulatory cells, such as CD4+Foxp3+ and CD4+LAP+, in the mesenteric lymph nodes. In conclusion, oral immunization with a single dose of poly(anhydride) nanoparticles loaded with cashew nut proteins leaded to a pro-Th1 and Treg immune response. Furthermore, their immunomodulatory properties could be introduced as a new approach for management of cashew nut allergy.
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