Use este identificador para citar ou linkar para este item:
https://www.arca.fiocruz.br/handle/icict/25887
COMPARATIVE GENOMICS OF THE ANOPHELINE GLUTATHIONE S-TRANSFERASE EPSILON CLUSTER
Sequência de Aminoácidos
Animais
Anopheles / enzimologia
Anopheles / genética
Anopheles / crescimento e desenvolvimento
Evolução Molecular
Éxons / genética
Fêmea
Duplicação Genética
Regulamento de Expressão Gênica, Desenvolvimento
Genômica / métodos
Glutationa transferase / química
Glutationa transferase / genética
Íntrons / genética
Modelos, Molecular
Dados de Sequencia Molecular
Família Multigene / genética
Filogenia
Conformação de Proteína
Seleção Genética
Especificidade de Espécies
Autor(es)
Afiliação
Liverpool School of Tropical Medicine. Vector Group. Liverpool, United Kingdom / Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.
Liverpool School of Tropical Medicine. Vector Group. Liverpool, United Kingdom / Swiss Tropical & Public Health Institute. Department of Medical Services and Diagnostics. Basel, Switzerland / University of Basel. Basel, Switzerland.
Liverpool School of Tropical Medicine. Vector Group. Liverpool, United Kingdom.
Liverpool School of Tropical Medicine. Vector Group. Liverpool, United Kingdom.
University of Liverpool. Institute of Integrative Biology. Liverpool, United Kingdom.
Liverpool School of Tropical Medicine. Vector Group. Liverpool, United Kingdom.
Liverpool School of Tropical Medicine. Vector Group. Liverpool, United Kingdom / Swiss Tropical & Public Health Institute. Department of Medical Services and Diagnostics. Basel, Switzerland / University of Basel. Basel, Switzerland.
Liverpool School of Tropical Medicine. Vector Group. Liverpool, United Kingdom.
Liverpool School of Tropical Medicine. Vector Group. Liverpool, United Kingdom.
University of Liverpool. Institute of Integrative Biology. Liverpool, United Kingdom.
Liverpool School of Tropical Medicine. Vector Group. Liverpool, United Kingdom.
Resumo em Inglês
Enzymes of the glutathione S-transferase (GST) family play critical roles in detoxification of xenobiotics across many taxa. While GSTs are ubiquitous both in animals and plants, the GST epsilon class (GSTE) is insect-specific and has been associated with resistance to chemical insecticides. While both Aedes aegypti and Anopheles gambiae GSTE clusters consist of eight members, only four putative orthologs are identifiable between the species, suggesting independent expansions of the class in each lineage. We used a primer walking approach, sequencing almost the entire cluster from three Anopheles species (An. stephensi, An. funestus (both Cellia subgenus) and An. plumbeus (Anopheles subgenus)) and compared the sequences to putative orthologs in An. gambiae (Cellia) in an attempt to trace the evolution of the cluster within the subfamily Anophelinae. Furthermore, we measured transcript levels from the identified GSTE loci by real time reverse transcription PCR to determine if all genes were similarly transcribed at different life stages. Among the species investigated, gene order and orientation were similar with three exceptions: (i) GSTE1 was absent in An. plumbeus; (ii) GSTE2 is duplicated in An. plumbeus and (iii) an additional transcriptionally active pseudogene (ψAsGSTE2) was found in An. stephensi. Further statistical analysis and protein modelling gave evidence for positive selection on codons of the catalytic site in GSTE5 albeit its origin seems to predate the introduction of chemical insecticides. Gene expression profiles revealed differences in expression pattern among genes at different life stages. With the exception of GSTE1, ψAsGSTE2 and GSTE2b, all Anopheles species studied share orthologs and hence we assume that GSTE expansion generally predates radiation into subgenera, though the presence of GSTE1 may also suggest a recent duplication event in the Old World Cellia subgenus, instead of a secondary loss. The modifications of the catalytic site within GSTE5 may represent adaptations to new habitats.
DeCS
3 'Regiões não traduzidas / genéticaSequência de Aminoácidos
Animais
Anopheles / enzimologia
Anopheles / genética
Anopheles / crescimento e desenvolvimento
Evolução Molecular
Éxons / genética
Fêmea
Duplicação Genética
Regulamento de Expressão Gênica, Desenvolvimento
Genômica / métodos
Glutationa transferase / química
Glutationa transferase / genética
Íntrons / genética
Modelos, Molecular
Dados de Sequencia Molecular
Família Multigene / genética
Filogenia
Conformação de Proteína
Seleção Genética
Especificidade de Espécies
Compartilhar