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ArticleCopyright
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Embargo date
2030-01-01
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- IOC - Artigos de Periódicos [12883]
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EFFECTS OF CARBON AND NITROGEN SOURCES ON THE PROTEOME OF PSEUDOMONAS AERUGINOSA PA1 DURING RHAMNOLIPID PRODUCTION
Proteômica
Fonte de carbono e nitrogênio
Ramnolipídio
Biorremediação
Author
Affilliation
Abstract
The PA1 strain of Pseudomonas aeruginosa isolated from oil waste produces rhamnolipid, a biodegradable
surfactant with applications in several industrial and environmental fields. The metabolic pathway and
genetic regulation of rhamnolipid production in P. aeruginosa are poorly understood. Herein, several proteins
directly or indirectly related to rhamnolipid production and their genetic regulations were identified
by comparative proteomic. We compared the proteome of P. aeruginosa PA1 after fermentation in two
different conditions of carbon and nitrogen sources: condition A allowed rhamnolipid production and
condition B prevented it. Protein extracts from cellular pellets were compared using 2D-PAGE stained
with colloidal Coomassie followed by MALDI-TOF/TOF mass spectrometry. We identified 21 differentially
expressed proteins, including those involved in secretion, quorum sensing, oxidative response and
metabolism.
Keywords in Portuguese
Pseudomonas aeruginosaProteômica
Fonte de carbono e nitrogênio
Ramnolipídio
Biorremediação
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