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2050-01-01
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ACTIVIN-A PROMOTES THE DEVELOPMENT OF GOAT ISOLATED SECONDARY FOLLICLES IN VITRO
Receptores de Activina Tipo II / genética
Activins / genética
Activins / farmacologia
Animais
Aromatase / genética
Células Cultivadas
Estradiol / análise
Estradiol / secreção
Fêmea
Regulação da expressão gênica / efeitos de drogas
Cabras
Técnicas de maturação de ovócitos in vitro / métodos
Folículo Ovariano / efeitos de drogas
Folículo Ovariano / fisiologia
Folículo Ovariano / ultraestrutura
Receptores FSH / genética
Autor
Silva, Cleidson Manoel Gomes da
Castro, Simone Vieira
Faustino, Luciana Rocha
Rodrigues, Giovanna Quintino
Brito, Ivina Rocha
Rossetto, Rafael
Saraiva, Márcia Viviane Alves
Campello, Claudio Cabral
Lobo, Carlos Henrique
Souza, Carlos Eduardo Azevedo
Moura, Arlindo de Alencar Araripe
Donato, Mariana Aragão Matos
Peixoto, Christina Alves
Figueiredo, José Ricardo de
Castro, Simone Vieira
Faustino, Luciana Rocha
Rodrigues, Giovanna Quintino
Brito, Ivina Rocha
Rossetto, Rafael
Saraiva, Márcia Viviane Alves
Campello, Claudio Cabral
Lobo, Carlos Henrique
Souza, Carlos Eduardo Azevedo
Moura, Arlindo de Alencar Araripe
Donato, Mariana Aragão Matos
Peixoto, Christina Alves
Figueiredo, José Ricardo de
Afiliación
Universidade Estadual do Ceará (UECE). Programa de Pós-Graduação em Ciências Veterinárias (PPGCV). Laboratório de Manipulação de Oócitos e Folículos Pré-Antrais (LAMOFOPA). Fortaleza, CE, Brasil.
State University of Ceará. Faculty of Veterinary Medicine. Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA). Fortaleza, CE, Brazil.
State University of Ceará. Faculty of Veterinary Medicine. Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA). Fortaleza, CE, Brazil.
State University of Ceará. Faculty of Veterinary Medicine. Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA). Fortaleza, CE, Brazil.
State University of Ceará. Faculty of Veterinary Medicine. Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA). Fortaleza, CE, Brazil.
State University of Ceará. Faculty of Veterinary Medicine. Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA). Fortaleza, CE, Brazil.
State University of Ceará. Faculty of Veterinary Medicine. Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA). Fortaleza, CE, Brazil.
State University of Ceará. Faculty of Veterinary Medicine. Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA). Fortaleza, CE, Brazil.
Federal University of Ceará. Department of Animal Science. Laboratory of Animal Physiology. Fortaleza, CE, Brazil.
Federal University of Ceará. Department of Animal Science. Laboratory of Animal Physiology. Fortaleza, CE, Brazil.
Federal University of Ceará. Department of Animal Science. Laboratory of Animal Physiology. Fortaleza, CE, Brazil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Laboratório de Ultraestrutura. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Laboratório de Ultraestrutura. Recife, PE, Brasil.
State University of Ceará. Faculty of Veterinary Medicine. Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA). Fortaleza, CE, Brazil.
State University of Ceará. Faculty of Veterinary Medicine. Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA). Fortaleza, CE, Brazil.
State University of Ceará. Faculty of Veterinary Medicine. Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA). Fortaleza, CE, Brazil.
State University of Ceará. Faculty of Veterinary Medicine. Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA). Fortaleza, CE, Brazil.
State University of Ceará. Faculty of Veterinary Medicine. Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA). Fortaleza, CE, Brazil.
State University of Ceará. Faculty of Veterinary Medicine. Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA). Fortaleza, CE, Brazil.
State University of Ceará. Faculty of Veterinary Medicine. Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA). Fortaleza, CE, Brazil.
State University of Ceará. Faculty of Veterinary Medicine. Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA). Fortaleza, CE, Brazil.
Federal University of Ceará. Department of Animal Science. Laboratory of Animal Physiology. Fortaleza, CE, Brazil.
Federal University of Ceará. Department of Animal Science. Laboratory of Animal Physiology. Fortaleza, CE, Brazil.
Federal University of Ceará. Department of Animal Science. Laboratory of Animal Physiology. Fortaleza, CE, Brazil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Laboratório de Ultraestrutura. Recife, PE, Brasil.
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Laboratório de Ultraestrutura. Recife, PE, Brasil.
State University of Ceará. Faculty of Veterinary Medicine. Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA). Fortaleza, CE, Brazil.
Resumen en ingles
The role of activin-A in follicular development and on the mRNA expression levels of different genes in goat secondary follicles was evaluated. Goat secondary follicles (≥ 150 μm) were cultured for 18 days under control conditions or with the addition of either 50 or 100 ng/ml activin-A (Experiment 1). The mRNA levels for the genes that code for activin-A, ActR-IA, ActR-IB, ActR-IIA, ActR-IIB, follicle stimulating hormone receptor (FSH-R) and P450 aromatase were measured in each condition (Experiment 2). We observed that after 6 days of culture, the antrum formation rate was higher in cultures with added activin-A than in the cultured control (P < 0.05). The addition of 50 ng/ml activin-A increased the follicular growth rate in the final third of the culture (days 12-18), resulting in a higher percentage of meiosis resumption (P < 0.05). On day 6, the addition of activin-A (50 ng/ml) increased the levels of ActR-IA mRNA compared with the cultured control (P < 0.05). After 18 days, the addition of 50 ng/ml activin-A significantly increased the levels of its own mRNA compared with the non-cultured control. Moreover, this treatment reduced the mRNA levels of P450 aromatase in comparison with the cultured control (P < 0.05). Higher levels of P450 aromatase mRNA were found for both activin-A treatments compared with the non-cultured control (P < 0.05). No difference in estradiol levels was detected among any of the tested treatments. In conclusion, the addition of activin-A to culture medium stimulated early antrum formation as well as an increase in the daily follicular growth rate and the percentage of meiosis resumption.
DeCS
Receptores de Activina Tipo I / genéticaReceptores de Activina Tipo II / genética
Activins / genética
Activins / farmacologia
Animais
Aromatase / genética
Células Cultivadas
Estradiol / análise
Estradiol / secreção
Fêmea
Regulação da expressão gênica / efeitos de drogas
Cabras
Técnicas de maturação de ovócitos in vitro / métodos
Folículo Ovariano / efeitos de drogas
Folículo Ovariano / fisiologia
Folículo Ovariano / ultraestrutura
Receptores FSH / genética
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