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https://www.arca.fiocruz.br/handle/icict/25014
SYNTHETIC PEPTIDES DERIVED FROM RIBOSOMAL PROTEINS OF LEISHMANIA SPP. IN MUCOCUTANEOUS LEISHMANIASIS: DIAGNOSTIC USEFULNESS
Diagnóstico
Péptido sintético
Proteína ribossômica
Parasita
Leishmania braziliensis
Diagnosis
Synthetic peptide
Ribosomal protein
Parasite
Leishmania braziliensis
Author
Affilliation
Universidad Nacional de Colombia. Facultad de Ciencias Grupo de Investigación en Inmunotoxicología. Departamento de Farmacia. Bogotá, Colombia
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Imunoparasitologia. Salvador, BA, Brasil
Pontificia Universidad Javeriana. Facultad de Ciencias. Grupo de Investigación en Enfermedades Infecciosas. Bogotá, Colombia
Universidad Católica de Valparaíso. Pontificia Curauma. Núcleo de Biolotecnología Curauma. Valparaiso, Chile
Instituto Nacional de Salud. Laboratorio de Parasitología. Bogotá, Colombia
Universidad del Rosario. Centro de Estudio de Enfermedades Autoinmunes. Bogotá, Colombia
Universidad Nacional de Colombia. Facultad de Ciencias Grupo de Investigación en Inmunotoxicología. Departamento de Farmacia. Bogotá, Colombia
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Imunoparasitologia. Salvador, BA, Brasil
Pontificia Universidad Javeriana. Facultad de Ciencias. Grupo de Investigación en Enfermedades Infecciosas. Bogotá, Colombia
Universidad Católica de Valparaíso. Pontificia Curauma. Núcleo de Biolotecnología Curauma. Valparaiso, Chile
Instituto Nacional de Salud. Laboratorio de Parasitología. Bogotá, Colombia
Universidad del Rosario. Centro de Estudio de Enfermedades Autoinmunes. Bogotá, Colombia
Universidad Nacional de Colombia. Facultad de Ciencias Grupo de Investigación en Inmunotoxicología. Departamento de Farmacia. Bogotá, Colombia
Abstract
The serological diagnostic methods currently available for mucocutaneous leishmaniasis (MCL) lack specificity when complete parasites are used; however, such specificity increases when protein fractions are used. Ribosomal proteins have been reported to induce antibodies in animal and humans infected with the parasite, making them a worth candidate to assess its diagnosis potential. Objective: This study was thus aimed at evaluating synthetic peptides derived from Leishmania
braziliensis ribosomal proteins S25 and S5 as antigen candidates for diagnosing MCL by ELISA
Methods: It was used 8 and 13 peptides derived from ribosomal proteins 25 and S5 respectively as
antigens in order to detect IgG antibodies by ELISA in people with active MCL, Chagas disease
(CH) and autoimmune disease (AID).
Results: 4 of these 21 peptides (P4, P6, P19 and P21) had the greatest sensitivity (21.7%, 13.04%,
20% and 20%, respectively) as well as having 95%, 100%, 100% and 82.5% specificity, respectively.
Conclusion: The study revealed the limited usefulness of the peptides being studied as a diagnostic
tool in the conditions used here, because its low sensitivity, but it is worth highlighting that the use
of peptides as antigen in the serodiagnosis of MCL may overcome the cross reaction presented with
other antigens, thus avoiding false positives.
Keywords in Portuguese
Leishmaniose mucocutâneaDiagnóstico
Péptido sintético
Proteína ribossômica
Parasita
Leishmania braziliensis
Keywords
Mucocutaneous leishmaniasisDiagnosis
Synthetic peptide
Ribosomal protein
Parasite
Leishmania braziliensis
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